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1'1' .E. T. Inc. <br />Water & Environmental Testine Inc. 479 West 50 North. American Fork U[ah 84003 (801)756-8397 <br />• <br />Acute Whole Effluent Toxicity Report <br />Ceriodaahreia <br />DATE: March 13, 1996 <br />PERMT['I'EE NAME: Wcstem Fucls-Colorado (New Horiwn Mine) NPDES NO: <br />TEST (AnimallAge): Ceriodaphnia <24 hours. <br />DATE/I'I11~fE TESL' BEGAN: 3/08/96 6:30 p.m. <br />TEST CONDTI'IONS <br />OUTFACE NO: 001 <br />SAMPLE (DatelType): 3/07/96. <br />DATF./P111fE TEST COMPLETED: 3/10/96 5:45 p.m. <br />Ceriodaphnia dubia neonates were exposed to diluted efllucnt as specified by permit. Survival al the end <br />of [be test period was measured and statistically evaluated for Acute Toxicity. <br />~J <br />Mimal Age. at Tesf.Start <24 hours.: I <br />Number of Organisms/Dilution Volume/Rcplicates 5 organisms/IS mI/4 replicates. <br />Food .:.: ' : YTC with.Algae(D.1. ml/day)(fed:previous.to.tesi):., <br />Aeration None required. <br />...... <br />'Dissolved Oxygen..:'.. ,. <br />Measured daily old/new::::.: ;, ;;;' ::.: : . . . .: .: :.: ';.::. <br />Water Replacement Renewed every 24 hours. <br />Tempecatrire . ;.. 20 + I degrees C: <br />Photo Period 16 hours ambient light/8 hours dark. <br />'.:...: . <br />casured iniually.and at , houi5..::....' ::. '. '.' .:. . <br />Dilution Water Reconstituted lab water (±15% hazdness of the <br /> Receiving Water). <br />. ' . <br />: <br />' le was <br />a1 Can oit':.(Sam supplied fo.:chclKUCa1::::::: <br />...... ... .. <br />stet :: <br />Receivi¢g.R <br />,:.:..: <br />,. <br /> <br /> <br />ysis but.not:used lotion}; ::.:::::' : ::. .:.:: :....:.:.:: <br />r <br />ana fo dt <br />Sample Concentrations Control, 6.25%, 12.5%, 25%, 50%, 100%. <br />SUMMARY <br />Results: ' X Pass Fail <br />There was NO significant effect on survival. <br />Enclosed aze data sheets and statistical reports. <br />Sincere) <br />~~ 1 GL4~`/ ~~ <br />Rawlings <br />Water & Environmental Testing, Inc. <br />Enclosure <br />• <br />