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., ~ 1'-' .E. T. Inc. <br />Wafer & Environmental Testine Inc. 479 West 50 North. American Fork Ulah 84003 (80/1756.8397 <br />• <br />Acute Whole Effluent Toxicity Report <br />Fathead Minrww <br />DATE: March 13, 1996 <br />PERMITTEE NAME: Western Fuels-Colorado (New Horizon Mine) NPDS NO: <br />TESL' (Anima!/Age): Fathead Mimow 13 days. <br />OUTFACE NO: 001 <br />SAMPLE (DatelType): 3/07/96. <br />DATE/TIME TESL' BEGAN: 3/08(96 7:00 p.m. DATE(TIl1TE TESL COMPLETED: 3(12/96 7:00 p.m. <br />TEST CONDti'IONS <br />Fathead Mimow larvae were exposed to diluted eftluenl as specified by permit. Survival at the end of the <br />lest period was measured and stalisticallyevalualed for Acute Toxicity. <br />• <br />;Anltnal.A~ge at.TesrStart 13 days. <br />Number o[ Organisms/Dilution Volume/Replicates 5 organisms/200 ml/4 replicates. <br />Fodd: Brine Shrimp.fed previoos io test and a1 48:hours:.::.::..:'. <br />Aeration Noue required. <br />:1)issolded: Oxygen :..:::.:: ..:::: : Measure: daily oldlnew: a: <br />Water Replacement Renewed every 24 hours. <br />...:::... . <br />Temperature '::.:::::::' .::: ::: <br />20 ±1::degrees.:C:.:' .:.:: ::.....:.::.::::..:::: ;:,;!.:!:...: <br />Photo Period 16 hours ambient light/8 hours dazk. <br />'pH-i `'` : ` :::::.. :: ::.:.:. .. Meastired:.iuititillynnd ati.24 hours:':::..:; ..;.:,,..:.:;::.. <br />Dilution Water Reconstituted lab water L15°/a hardness of the <br /> Receiving Water). <br /> <br />lyirtg..Water ::. .. .:.: .::...:.:::... <br />Rece . . <br />:.::....:.:......::..... ;:-: <br />;..:.... <br />Con Gan on (Sam le wis supplied.for cheaucal " <br />analysis.but noi,psed foi;diluUogwater):.':.:;>::.:::::I:;'.:i. <br /> <br />•: <br />Sam le Concentrations <br />P Control, 6.25%a, 12.5%, 25%a, 50%, 100%. <br />SUMMARY <br />Results: }C Pass Fail <br />There was NO significant effect on survival. <br />Enclosed are data sheets and statistical reports. <br />~ ~~ <br />C~J !~v \ <br />- / <br />Water & Environmental Testing, lnc. <br />Enclosure <br />• <br />