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. SSA <br />n <br />LJ <br />U <br />Narrative and Discussion <br />ADDRESS ALL CORRESPONDENCE TO: <br />COMMERCIAL TESTING 8 ENGINEERING CO. <br />4665 PARIS STREET, SUITE R-200 <br />DENVER, CO 80239 <br />TEL: (303( 373-0772 <br />FAX: (303) 373-d 791 <br />PERMIT AND SAMPLE INFORMATION <br />NPDES permit #: <br />Type of sample: <br />Date and time of sample(s): <br />Date and time of test start: <br />Dilution water. <br />Receiving temperature(s): <br />CO-0000213 <br />Effluent Grab <br />03/02104 1040 <br />03/03/04 1330 <br />Reconstituted Laboratory Water <br />-2.0 ° C <br />• TEST ORGANISMS <br />Fathead minnow <br />Daphnia magna <br />Age: 9 days <br />Age: <24 hrs <br />. PROCEDURE <br />Acute, 48 and 96-hour bioassays as outlined in the most recent protocols specified by the Water <br />Quality Control Division of the Colorado Department of Health (1998). <br />DISCUSSION <br />According to the Western Fuels -Colorado discharge permit (CO-0000213), an acute WET test is <br />failed whenever the LCD, which represents an estimate of the effluent concentration which is <br />lethal to 50°J° of the test organisms in the time period prescribed by the test, is found to be less <br />than or equal to 100% effluent. <br />By this definition, the results of this bioassay indicate that effluent from outfall 007 is not acutely <br />toxic to fathead minnow and Daphnia magna survival. Therefore, outfall 007 is in compliance <br />with the acute WET portion of its Colorado State Industrial General Minimal Discharge Permit for <br />the first quarter of 2004. <br />DATE OF REPORT <br />March 9, 2004 <br />Rep rt prepared by: Sarah Mozingo <br />/~ <br />B monitoring Sup iso Dat <br />Report reviewed by: Heath Smith <br />3-lo-oy <br />Labora ory Technician Date <br />fnmr,~e~oal les;uc; Y. l rvaneo-nry fn Minerals Services- CarpOrale Office <br />1919 S. Highland Ave., Suile 2108. Lombard. IL 60148 t 16301953-9300 f 16301953-9306 vnvwsgs corn <br />s-sa ' Memeer pl me sGS Gmup ISavitte GMtraie ae Sune~nmcel <br />