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.~ <br />Table 1 -Colorado Acute Test Conditions <br />ADDRESS ALL CORRESPONDENCE TO: <br />COMMERCIAL TESTING 8 ENGINEERING CO. <br />4665 PARIS STREET, SUITE 8-200 <br />DENVER,CO 80239 <br />TEL: (303) 373-0772 <br />fAX: (303) 3734791 <br />Parameter <br />Test Type <br />Temperature <br />Photoperiod <br />Test Chamber Size <br />Test Sample Volume <br />Renewal Period <br />Age of Organisms <br />Number per Chamber <br />Chambers Per Concentration <br />Feeding Regime Per Chamber <br />Test Duration <br />C. dubia & D. magna Fathead Minnow <br />Static Renewal Static Renewal <br />20 +/- 1 ° C 20 +/- 1 ° C <br />16 hr IighU8 hr dark 16 hr IighU8 hr dark <br />30 ml 600 ml <br />20 ml 300 ml <br />24 hrs 24 hrs <br />< 24 hrs 1 to 14 days <br />5 10 <br />4 4 <br />0.1 ml Algae/YTC 0.1 ml Brine Shrimp <br />48 hrs 96 hrs <br />Table 2 -Methods and Equipment <br />• Characteristic <br />Alkalinity <br />Ammonia, as Nitrogen <br />Chlorine, Total Residual <br />Conductivity <br />Dissolved Oxygen <br />Hardness <br />pH <br />Temperature <br />References <br />Method/Equipment EPA Method <br />Titrimetric (APHA6, 1989) <br />Colorimetric Analysis Method <br />laMotte STC-Cl Colorimeter <br />Oakton CON 500 Cond. Meter <br />Orion Model 850 DO Meter <br />Titrimetric (APHA, 1989) <br />Fisher Model 925 pH Meter <br />NIST' calibrated thermometer <br />EPA 310.1 <br />EPA 350.1 <br />EPA 330.4 <br />EPA 120.1 <br />EPA 360.1 <br />EPA 130.2 <br />EPA 150.1 <br />EPA 170.1 <br />American Public Health Association. 1992. Standard Methods for the Examination of Water and <br />Wastewater, 18'" Edition. American Public Health Association, Washington, D.C. <br />Colorado Department of Health. 1998. Guidelines for Conducting Whole EfFluent Toxicity Tests. Water <br />Quality Control Division, Denver, Colorado 80246-1530. <br />USEPA. 1993. Methods for Measuring the Acute Toxicity of Effluents to Freshwater and Marine <br />Organisms. EPA/600/4-90/027F, 4th edition. EMSL, Cincinnati, Ohio 45268. <br />n <br />LJ <br />c American Public Health Association <br />National Institute of Standards and Technology <br />S .~d~cgno idl l~sbcj P. ingr,ier:•-i; I,i, Nh°EraIS $CMC2S-COfpplale Rltice <br />1919 S. Highland Ave.. Swte 210& Lombard. IL 60148 t 16301953-9300 f (6301953-9306 wvavsgs.com <br />Faxs T Mempe~ of tie SGS G*ouo lSxlNk GQnE+ele de Surveluancel <br />