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Narrative and Discussion <br />PERMIT AND SAMPLE INFORMATION <br />TEST ORGANISMS <br />Ceriodaphnia dubia <br />Age: <24 hrs <br />. PROCEDURE <br />Acute, 48-hour bioassay as outlined in the most recent protocols specified by the Water Quality <br />Control Division of the Colorado Department of Public Health and Environment (1998). <br />NPDES permit #: <br />Type of sample: <br />Date and time of sample(s): <br />Date and time of test start: <br />Dilution water. <br />Receiving temperature(s): <br />ADDRESS ALL CORRESPONDENCE <br />COMMERCIAL TESTING 8 ENGINEERING CO. <br />4665 PARIS STREET, SUITE &200 <br />DENVER, CO 60239 <br />TEL: (303) 3734772 <br />PAX: (303) 3734791 <br />CO-0032115 <br />Effluent Grab <br />07/21/03 0900 <br />07/22/03 1350 <br />Reconstituted Laboratory Water <br />16.0 ° C <br />• <br /> <br />DISCUSSION <br />According to the Trapper Mining lne. discharge permit (CO-0032115), an acute WET test is failed <br />whenever the LCSD, which represents an estimate of the effluent concentration which is lethal to <br />50% of the test organisms in the time period prescribed by the test, is found to be less than or <br />equal to '100% effluent. <br />By this definition, the results of this bioassay indicate that effluent from outtall 002 is not acutely <br />toxic to Ceriodaphnia dubia survival. Therefore, outtall 002 is in compliance with the acute WET <br />portion of its Colorado State Discharge Permit for the third quarter of 2003. <br />DATE OF REPORT <br />July 24, 2003 <br />• <br />Report prepared. bv: Chris Theel <br />Cj~,t~.... ~~ 7?S-°.3 <br />Biomonitoring Supervisor Date <br />Re port reviewed by: Sarah Lohner _ <br />/ ~~ <br />Laboratory Technician ate <br />Commercial Te:uno S Emm~eenrc G°. Mineral: Services -Corporate Office <br />1979 S. Hi°hland Ave., Suite 2108, Lombard, IL 60148 t 163DI953-9300 t 163019539306 <br />• <br />Memhe~ of the SGS Group IGOCiere G¢neeale tle Gurvelllencel <br />