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segment sprouts, and potted transplants at Seneca II-W primary study plot and the <br />additional fenced site; and monitoring of the native sprouts on the unmined site at <br />Yoast. These measures are largely addressed under the Proposed Future Research <br />section of the pilot study report, and will just need to be fleshed out in detail in the <br />2na year study plan. The plan should also include commitment to submittal of a <br />longer term study plan by December 2006. <br />2. Competing vegetation was rigorously and continuously controlled by mechanical <br />"weeding" within the fenced primary study plot during the initial fteld season. <br />This level of competition suppression would not be realistic under operational <br />field conditions, nor would it be desirable once transplants are adequately <br />established, since associated shrubs and forbs are typically planted and seeded in <br />aspen establishment areas, in addition to the aspen transplants. Fabric weed <br />barrier product was used in conjunction with the commercial nursery stock <br />planted in the additional fenced plot described in the study, but no further control <br />of competition was conducted. I recommend that the 2"a year study plan <br />incorporate suppression of competing vegetation as an additional study <br />variable. <br />Slides projected during Dr. Shepperd's presentation showed minor salt <br />accumulations at the soil surface in the imgated treatments. The researches <br />indicated that physiological data showed no indication of osmotic effects on aspen <br />growth. However, it was noted that the irrigation water source is a sediment pond <br />that receives a component of inflow from relatively saline spoil spring discharge. <br />Under dryer growing season conditions than prevailed in 2005, it is possible that <br />osmotic effects on growth of imgated transplants might be expressed. I <br />recommend that this factor be considered, and that the 2"a year study plan <br />include proposal to periodically monitor irrigation water salinity <br />(conductivity) and root zone saturated paste extract conductivity throughout <br />the growing season. <br />4. Given the reported differences with respect to root segment sprouting, sapling <br />survival and growth, and physiological status of aspen in the "fresh" or <br />"modified" soil treatment as compared to the "stored" or "normal" soil treatment, <br />and the uncertainty regarding which factors may account for the observed <br />differences, the 2na year study plan should include a plan for soil sampling and <br />analysis within each of the soil treatments in the primary study plot, as well as the <br />additional fenced planting area at Seneca II-W. At a minimum, the plan should <br />include procedures for representative sampling and analysis of soil texture <br />~ and fertility (organic matter,~N,P,K) by I foot depth increments within each <br />soil treatment. PH, <br />In conclusion, although there were aspects of the pilot study that diverged somewhat <br />from the original study plan, initial results are in general quite encouraging. <br />Continuation of the study for a second season and for an as yet undetermined longer <br />