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2, The study was properly expanded to incorporate growth and survival data <br />collection for non-imgated root segment sprouts that developed on the two soil <br />treatments within the fenced primary study plot, as well as commercially grown <br />aspen seedlings that were planted in a nearby fenced area at Seneca II-W, and <br />natural sprouts located on a surface disturbance area at Yoast that had not been <br />mined. Additional documentation should be included in the report, regarding <br />certain aspects of the expanded study. Please amend the report to include: <br />• A map or maps at appropriate scale to show the location of the <br />primary study site at Seneca II-W, the nearby fenced site at II-W, and <br />the monitored site at Yoast. <br />• A description of the soil treatment for the commercial seedling <br />planting site, as requested for the primary study site soil treatments. <br />• Details on potted nursery stock, including size of the transplants, <br />type/size of container, site origin of the seedling stock <br />(Forest/county/elevation, etc.), planting timing and method, and any <br />post-planting cultural practices (irrigation, weed control, etc.). <br />Proposed Future Research <br />The report prepared by the RMRS researchers contains proposals for future study, but at <br />this point there are no specific commitments, beyond those included in the permit <br />Appendix 22-3 study plan referenced earlier in this memorandum. Based on the initial <br />relatively high survival observed for various treatments, including native transplants, root <br />segment sprouts, and commercial nursery seedlings, it is clear that a continuation of the <br />study is warranted in order to obtain meaningful results on longer term growth, survival, <br />and imgation response. Permit Appendix 22-3 indicates that additional study plans <br />would be submitted no later than December 2006, with level of irrigation to continue <br />through the 2006 field season at levels to be determined by 2005 study results. However, <br />it would seem that a more detailed second year study plan addressing evaluation of <br />survival, growth, and physiological status, in addition to irrigation treatments, should be <br />formalized and submitted as soon as practicable. By December 2006, a plan for longer <br />term research should be submitted. Ideally, to ensure proper continuity of the study, the <br />RMRS involvement in monitoring, assessment, and reporting would continue through the <br />2"d year study, as well as future longer term research. My specific recommendations <br />regarding future research include: <br />A 2"d year study plan should be submitted as a minor revision to the permit, <br />by April 15, 2006. The revision might logically be submitted as an addendum to <br />the Appendix 22-3 study plan. The plan should include 2"d year irrigation <br />treatment rates and frequency, and should address maintenance of precipitation, <br />soil moisture, and temperature monitoring systems in the primary study plot; <br />quality assurance/quality control procedures, protocol for plot management and <br />monitoring of survival, growth, and physiological status of native transplants, root <br />