Laserfiche WebLink
- 13 - <br />d) the liquid was centrifuged at high speed for 20-30 minutes in order to <br />concentrate the bacteria cells; <br />e) the cells were resuspended in a sulfuric acid solution at pH 2; <br />f) the final solution was re-examined under the microscope for viable <br />bacteria. <br />An equal volume of sterile acid solution was added to the control <br />non-innoculated samp$s (A1 and A3) at the sixth week of leaching. <br />At the conclusion of the humidity cell test, the leachates from <br />the two innoculatzd units were analyzed for the presence or absence of <br />Thiobacillus ferrooxidans according to a method described by Silverman et. <br />al. (25). <br />Particle Sizes. A sample of waste rock from the south <br />tails area of the Equity Mine was crushed to four particle size ranges <br />(sample 'B1 to 64 in Table 2). Samples were collected for each particle <br />size range from the appropriate sieve and placed into the humidity cell <br />apparatus for testing as previously described. <br />Blending. Two experiments were conducted to determine <br />the effect of blending a potential acid producing (A1) and an acid consum- <br />ing rock (C3). Sample D1 contained 20% sample Al and 80% sample C3 for a <br />net neutralization potential of -3 tonnes CaC03/1000 tonnes. The second <br />sample, 02, contained 10% Al and 90% C3 for a net neutralization potential <br />of +51 tonnes CaC03/1000 tonnes. Blended samples were thoroughly nixed and <br />placed in the humidity cells for leaching. <br /> <br />