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1 <br />1 <br />1 <br />1 <br />1 <br />1 <br /> <br />Standard Operating Procedure <br />Sample Filtration <br />1.0 Equipment <br />• Nalgene disposable filter apparatus <br />Polyethylene transfer bottle <br />• Hand vacuum pump <br />• Tygon tubing <br />2.0 Filtering procedures <br />1. Assemble the filter. <br />2. Pour the sample into the top portion of the filter. <br />3. Attach vacuum pump to filter apparatus. <br />4. Apply vacuum suction through filter by hand pumping for sample transfer to bottom <br />chamber. <br />5. Pour the filtered sample from the bottom chamber of the filter directly into the sample <br />containers. <br />6. Dispose of the used filter apparatus. <br />