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PERMIT, PART I PegC I f of 13 Permit Nu. CO-0032G38 The permittee shall conduct each chronic WET test in general accordance with methods described Short Term Methods for E.stimatine the Chronic Tonicity of Effluents and Receivine Waters to Freshwater Oreanisms, EPA/G00/4-89/001 or most current editions, except as modified by the most current Division guidance document eatitled Guidelines for Conducline Whole Effluent Tonicity Tests..Tbe permittee shall conduct such tests using Ceriodaphnia dubia aad fathead mimows. (b) Failure of Test and Divisioa Notification (Prior to Effective Date of Limitation) Prior to the effective date of the limitation, a chronic WET test is failed whenever there is a statistically significant difference in lethality between the control aad any effluent concentration less than or equal to the ins4eam waste oonceatration ("IWC') and the IC25, which represents m estimate of tite effluent concentration at which 2596 of the test organisms demonstrate inhibition as reflected by lethality, is at any effluent wacentration less than or equal to the IWC. The IWC for this permit has been determined to be 34%. The permittee must provide written notification of the failure of a WET test to the Divisioa, along with a statement as to whether a Preliminary Tonicity Investigation ("PTI')/1'oxicity Identification Evaluation ("TIE") or accelerated testing is being performed. NotJcatioa must be received by the Division within 21 calendar days of the demonstration of chronic WET In the routine required test. "Demonstration" for the purposes of Parts I.B.4(b),(c),(d) and (t) mesas no later than the last day of the laboratory test. (c) Failure o_f_Tnst and Divisioa Notification (Beginnine on Effective Nate of Limitation) Beginning on the effective date of the limitation, a chronic WET test is failed whenever there is a statistically sigrtificant difference in lethality betwew the control and any effiueat concentration less than oc equal to the isci.,.s... waste concentration ("IWC"). The IWC for this permit has bees determined to be «"'. The permittee must provide written notification of the failure of a WET test to the Division, along with a statement as [o whether a Preliminary Toxicity Investigation ("PTI")/Toxicity Identification Evaluation ("TIE") or accelerated testing is being performed. Notification must be received by the Division within 21 calendar days of the demonstration of chronic WET 1n the routine trequrred test. "Demonstration" for the purposes of Parts I.B.4(b),(c),(d), (e) and (g) means no later man the last day of the laboratory test. (d) Automatic Comoliaoce Schedule Upon Failure of Test !f a routine chronic WET test is failed, regardless of whether the limit is in effect, the following automatic compliance schedule shall apply. As paR of this the permittee shall tither: G) P~~ ~ conduct the 1''I7/TTE investigation as described in PaR I.B.4.e, or (ii) conduct accelerated testing using the single apxics found to be more sensitive. If accelerated testing is being performed, the permittee shall provide written notifigtion of the results within 14 calendar days of completion of the "Pattern of Toxicity"/"Na Toxicity" demonstration. Testing will be at least once every two weeps for up to five tests until; 1) two consecutive tests fail or three of five tests Coil, in which case a pattern of toxicity has been demonstrated or 2) two oortsxutive tests pass or three of five tesu pass, in which case no pattern of toxicity has been found. If no pattern of toxicity is found the toxicity episode is considered to be ended and routine testing is to resume. If a pattern of toxicity is found, a PTUTTE investigation is to be performed. If a pattern of tonicity is not demonstrated but a sigrvficant level of erratic toxicity is found, the Division may require an increased frequency of routine monitoring or some other modified approach.