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<br />I' 1 <br />LJ <br />• <br />COMMERCIAL TESTING 8 ENGINEERING CO. <br />METHODS <br />A two species acute bioassay was conducted with et~luent from the Lone Pine Pipeline, outfall <br />013. An effluent gab sample was collected on October 1, 1999, stored on ice, and delivered to <br />Commercial Testing and Engineering, Co. (CT&E) on October 4, 1999. The effluent sample was <br />received at a temperature of 8.0 ° C and assigned CT&E sample tracking number 72-414613. <br />The bioassay was initiated approximately 72 hours after sample collection. <br />Testing was conducted in accordance with the most recent protocols specified by the Water <br />Quality Control Division of the Colorado Department of Health (1998), outlined in Table I . <br />Basic characteristics of the effluent and dilutions were measured as follows: <br />Characteristic Method/Equipment EPA Method <br />Alkalinity <br />Ammonia <br />Chlorine, Total Residual <br />Conductivity <br />Dissolved Oxygen <br />Hardness <br />pH <br />Temperature <br />Titrimetric (APHAI, 1989) <br />Selective Ion Electrode Method <br />LaMotte STC-CI Colorimeter <br />Orion Model 160 Cond. Meter <br />Orion Model 850 DO Meter <br />Titrimetric (APHA, 1989) <br />Fisher Model 925 pH Meter <br />NISTZ calibrated thermometer <br />EPA 310.1 & 130.2 <br />EPA 350.3 <br />EPA accepted <br />EPA 120.1 <br />EPA 360.1 <br />EPA 310.1 & 130.2 <br />EPA 150.1 <br />EPA accepted <br />The tests exposed daphnids, Daphr:ia magna, to five concentrations of ef'Fluent, 100%, 75%, <br />50%, 25%, and 12.5%, prepared using reconstituted laboratory water at 170 mg/L CaCO~. <br />Additionally, the tests exposed fathead minnows, Pimephales promelas, to five concentrations of <br />etlluent, 100%, 75%, 50%, 25%, and 12.5%, prepared using reconstituted laboratory water at <br />120 mg/L CaC03. Control replicates were exposed to 100% laboratory water. <br />The Binomial method determined the LCS°'s. Significant differences in mortality were analyzed <br />using the Steel's Many-One Rank tests. <br />' American Public Health Association <br />National Insutute of Standards and Technology <br />F OB° Lt! <br />Original Watermarked For Vour Protection <br />