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METHODS Contra <br />Solid Phase Extraction/pH Adjustment Test (pH 3, pH,) - <br />Effluent at pH 3 and initial pH from the pH adjustment <br />and filtration test were passed through C1B octadecyl <br />Solid Phase Extraction (SPE) columns. Column flow was <br />reverse gravity at 5 ml/min with a FMI Fluid Metering <br />Pump. Prior to organism exposure, pH adjusted samples <br />were adjusted to the initial pH with NaOH and HZSOa. A <br />total of ten organisms were exposed to 0$, 12.5$, 50$ and <br />100$ dilutions prepared using reconstituted laboratory <br />water at 125 mg/L CaC03. <br />Oxidant Reduction Test - Ten organisms were exposed to <br />each of 20 milliliter samples of 100$ effluent aliquots <br />containing 0.0 ml, 0.012 ml, 0.025 ml, 0.05 ml, 0.1 ml, <br />0.2 ml, 0.4 ml, 0.6 ml, 0.8 ml and 1.0 ml of a 22 g/L <br />Sodium Thiosulfate (NaZSZ03) stock solution. Time to <br />mortality was observed beginning approximately 24 hours <br />after initial exposure. <br />EDTA Chelation Test - Ten organisms were exposed to each <br />of 20 milliliter samples of 1003 effluent aliquots <br />containing 0.0 ml, 0.012 ml, 0.025 ml, 0.05 ml, 0.1 ml, <br />0.2 ml, 0.4 ml, 0.6 ml, 0.8 ml and 1.0 ml of a 10 g/L <br />EDTA stock solution. Time to mortality was observed <br />beginning approximately 24 hours after initial exposure. <br />5 <br />COMMERCIALTESTING & ENGIN EE RING CO. <br />F466 L~~ <br />Original Warartnarked For Vour Pralection ., <br />