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METHODS Cont ~ d pH Adjustment Test (pH 3, pHl, pH 11) - Ten organisms were exposed to 0$, 12.5$, 50$ and 100$ effluent, 0$, 12.5$, 50$ and 100$ effluent adjusted to pH 3 with Sulfuric Acid (HZSO4), and 0$, 12.5$, 50$ and 100$ effluent adjusted to pH 11 with Sodium Hydroxide (NaOH). Prior to organism exposure, effluent adjusted to pH 3 was returned to the initial effluent pH with NdOH. After settling for one hour, and prior to exposing organisms to the sample, the pH 11 effluent was adjusted back to the initial pH with HCL. Dilutions were prepared with reconstituted laboratory water at 125 mg/L CaC03. Control organisms were exposed to reconstituted laboratory water adjusted to pH 3 and pH 11 with the same stock acid and base as above. Graduated pH Test (pH 6.0, pH 7.0, pH 8.0) - Ten organisms were exposed to effluent adjusted to pH 6.0, pH 7.0 and pH 8.0. Effluent pH was adjusted with HZSOa and/or NaOH. Tests were conducted so as to limit airspace in test cups in an effort to control pH drift. Filtration/pH Adjustment Test (pH 3, pH„) - Ten organisms were exposed to 0$, 12.5$, 50$ and 100$ whole effluent and 0$, 12.5$, 50$ and 100$ effluent at pH 3, following filtration through a 1.2 micron glass fiber filter and adjustment to the initial effluent pH. Effluent pH adjustments were performed with HZSOy and NaOH. Dilutions were prepared with reconstituted laboratory water at 125 mg/L CaCO3. Aeration/pH Adjustment Test (pH 3, pHl,) - Ten organisms were exposed to aliquots of effluent aerated at the initial pH, and pH 3. Samples were aerated for approximately six hours. Following aeration, and prior to exposing the organisms to 0$, 12.5$, 50$ and 100$ dilutions, the samples were adjusted to the initial pH with HZSO, and NaOH. Dilutions were prepared with reconstituted laboratory water at 125 mg/L CaC03. 4 COMMERCIALTESTING 8r ENGIN EE RING CO. Fd66 L~~ Original Watermarkatl For Vour protection ,