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_ ~ PART I Page lg of 19 Permit No. CO-0000132 B. MONITORING REQUIREMENTS 5. Accelerated Testing - Discharge point 011 If an individual toxicity test shows toxicity at concentrations less than or equal to the Instream Waste Concentration (IWC) of 1% or if SOX species mortality in any dilution is detected in any acute WET test conducted in accordance with this permit, the permittee shall notify the Division verbally within 24 hours and in writing within 5 days of becoming aware of the toxicity. Within 7 days of becoming aware of the toxicity, the permittee shall then begin accelerated testing of the discharge with whichever species has proven (through the first instance of toxicity) to be the most sensitive. The permittee will continue such testing with one species, once every week until directed by the Division to do otherwise, but in no case for more than five representative tests. 6. Preliminary Toxicity Investigation If directed by the Division, the permittee shall perform a preliminary toxicity investigation. The permittee will have 15 working days from the date of Division notification to complete a preliminary toxicity investigation and submit the results to the Division, in writing. The preliminary toxicity investigation may include, but is not limited to: additional chemical and biological monitoring, examination of pretreatment program records, examination of discharge monitoring reports, evaluation of treatment processes and chemical use, inspection of material storage and transfer areas to determine if a spill may have occurred, and similar procedures. If the preliminary toxicity investigation identifies a probable toaicant and/or a probable source of toxicity, the permittee shall submit, as part of its final report results, written notification to that effect to the Division. Within 30 days of completing the preliminary tonicity investigation, the permittee shall submit for the Division's approval a control program to control effluent toxicity, and shall proceed to implement such plan within 7 days following approval by the Division. If no probable explanation for toxicity is identified in the 15-day preliminary toxicity investigation, a Phase I Toxicity Reduction Evaluation (TRE) is required. The permittee shall notify the Division, as part of its final report, of its intent to proceed with the Phase I TRE. If tonicity disappears during the preliminary toxicity investigation, the permittee shall submit written notification to that effect to the Division. The Division may require the petmittee to conduct accelerated testing to demonstrate the continued absence of toxicity. If no acute toxicity is found to exist, the toxicity incident is closed.