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SGS <br /> AOOHESS AlL CGRHESPONDENf,E TO <br />COMMEHCIAI rESt MG 8 ENG~NEFHING CO <br /> +665 PAH~5 S1rIF.Ff t,:I1NF B~)W <br /> UE NVL N.CU 1%11?9 <br /> fEl ;:i0:n ?1:5~d/T7 <br /> iAY 1T:x3151:1+191 <br />Table 1 -Colorado Acute Test Conditions <br /> <br />Parameter C. dubia 8 D. magna Fathead Minnow <br />Test Type Static Renewal Static Renewal <br />Temperature 20 +1- 1°C 20 +!- 1`C <br />Photoperiod 16 hr IighUB hr dark 16 hr IighUB hr dark <br />Test Chamber Size 30 ml 600 ml <br />Test Sample Volume 20 ml 300 ml <br />Renewal Period 24 hrs 24 hrs <br />Age of Organisms < 24 hrs 1 to 14 days <br />Number per Chamber 5 10 <br />Chambers Per Concentration 4 4 <br />Feeding Regime Per Chamber 0.1 ml Algae/YTC 0.1 ml Brine Shrtmp <br />Test Duration 48 hrs 96 hrs <br />Table 2 -Methods and Equipment <br />Characteristic MethodlEquipment EPA Method <br />Alkalinity Titrimetric (APHA`, 1989) EPA 310.1 <br />Ammonia, as Nitrogen Colorimetric Analysis Method EPA 350.1 <br />Chlorine. Total Residual LaMotte 5TC-CI Colorimeter EPA 330.4 <br />Conductivity Oakton CON 500 Cond. Meter EPA 120.1 <br />Dissolved Oxygen Orion Model 850 DO Meter EPA 360.1 <br />Hardness Titrimetric (APHA, 1989) EPA 130.2 <br />pH Fisher Model 925 pH Meter EPA 150.1 <br />Temperature NIST7 calibrated thermometer EPA 170.1 <br />References <br />American Public Health Association. 1992. Standard Methods for the Ezamfnabon o/ Water and <br />Wastewater. 18'" Edition. American Public Health Association, Washington, D. C. <br />Colorado Department of Health. 1998. Guidelines for Conducting Whole Eff9uent ToxicRy Tests. Wa <br />Quality Control Division, Denver, Colorado 80246-1530. <br />USEPA. 1993. Methods for Measuring the Acute Toxicity of EKluents to Freshwater and Marine <br />Organisms. EPA1600/4-90;027F, 4'" edition. EMSL, Cincinnati, Ohio 45268. <br />American Public floalth Association <br />National Instihlfe nl Standards and Toehnobgy <br />ehf Awe .Sala 11IMf. taMnlL 1160148 t 10701 fi7~9:100 1163f)195]93{~ Aww sy~ cum <br />___-.- MYn~-tlI1M 3fiG 4rou+x$otluGWxW Y. SnrvexNnt~ <br />