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SGS <br />Narrative and Discussion <br />ADDRESS All CORRESP(N,DENCE IQ <br />NfAEPCIAt 1ESTING A @NGINFE NIrI(i C,iJ <br />JBfu EAR'S STREET ':VIT! &IC%1 <br />f AH UiC•1 NJ-07bi <br />PERMIT AND SAMPLE INFORMATION <br />MPDES permit t: CO-0000213 <br />Type of sample: E111uent Grab <br />Date and time of sample(s): 03102104 1040 <br />Date and time of lest start: 03103/`04 1330 <br />Dilution water: Reconstituted Laboratory Water <br />Receiving temperature(s): -2.0 ° C <br />TEST ORGANISMS <br />Fathead minnow Age: 9 days <br />Da~,hnia magna Age' <24 hrs <br />. PROCEDURE <br />Acute. 48 and 96-hour bioassays as outlined in the most recent protocols specified by the Water <br />Quality Control Division of the Colorado Department of Health (1998). <br />DISCUSSION <br />According to the Western Fuels -Colorado discharge permit (CO-0000213), an acute WET test is <br />failed whenever the LC;~, which represents an estimate of the effluent concentration which is <br />lethal l0 50% of the test organisms in the time period prescribed by the test, is found to be less <br />than or equal to 100% effluent. <br />By this definition, the results of this bioassay indicate that effluent from outfalt 007 is not acutely <br />Toxic to fathead minnow and Daphnia magna survival. Therefore, outfall 007 is in compliance <br />with the acute WET portion of its Colorado Stale Industrial General Minimal Discharge Permit for <br />the first quarter of 2004. <br />DATE OF REPORT <br />March 9. 2004 <br />Rep rt prepared by: Sarah Mozingo Report reviewed b Heath Smith <br />~. __ 3-lo-oy <br />1~~ +I <br />~. <br />monitoring Su/p iso Da~ Labora cry Techrnctan Date <br />