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Mountain Coal Company CO - 0038766 SCG Project No.: 304222 <br />Site: West Elk Mine <br />Acute Toxicity Test Summary <br />Test; • 48-hour static renewal using Daphnia magna <br />• 96-hour static renewal using Fathead minnows (Pimephales <br />promelas) <br />Client: Mountain Coal <br />Test Procedure Followed: EPA-821-R-02-012 <br />Sample Description: 304222 <br />Time of Sample Date of Sample Time of Sample Date of Sample <br />Collection Collection Recei t Receipt <br />0900 8-25-04 1020 8-26-04 <br />"~ aphnia agna ahead innows ~"' <br />Test Initiation Time 1230 1145 <br />Test Initiation Date 8-26-04 8-26-04 <br />Test Completion Time 1210 1045 <br />Test Completion Date 8-28-04 8-30-04 <br />Dilution Water: • Moderately hard laboratory reconstituted water <br />Test Organism Source: • Daphnia magna <br />The SeaCrest Group <br />• Fathead minnows <br />The SeaCrest Group <br />Reference Toxicant: • Sodium Chloride <br />The SeaCrest Group 3 <br />