HYDRO27761

• Permit No: C1980007
• IBM Index Class Name: Hydrology
• Doc Date: 11/1/2004
• Doc Name: Biomonitoring Report Dated September 22, 2004
• From: SeaCrest Group
• To: Mountain Coal Company
• Permit Index Doc Type: DMR’s
• Media Type: D
• Archive: No

Mountain Coal Company CO - 0038766 SCG Project No.: 304222
Site: West Elk Mine
TABLE OF CONTENTS
ACUTE TOXICITY TEST SUMMARY .................................................................................... 3
ABSTRACT OF RESULTS ......................................................................................................... 4
CONTROL .................................................................................................................................... 4
INTRODUCTION ......................................................................................................................... 5
MATERIALS AND METHODS ................................................................................................. 5
SAMPLE COLLECTION .................................................................................................................. $
SOURCE AND ACCLIMATION OF TEST ORGANISMS ....................................................................... $
TEST PROCEDURES ....................................................................................................................... $
TEST PROCEDURES FOR DILUTED ACUTE TESTS .......................................................................... 6
DATA ANALYSIS .......................................................................................................................... 6
RESULTS ...................................................................................................................................... 7
DISCUSSION ................................................................................................................................ 7
REFERENCES .............................................................................................................................. 7
APPENDIX 1-CHAIN OF CUSTODY FORM ....................................................................... 8
APPENDIX 2 -LAB DATA SHEETS ........................................................................................ 9
APPENDIX 3 - QA/QC AND REFERENCE TOXICANT TEST CHART .........................12
LIST OF TABLES
TABLE 1 -Daphnia magna Survival and LC50 Calculations .......................................7
The SeaCrest Group 2