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W.E.T. Inc. III IIIIIIIIIIIII III <br />Water & Environmenfa! Teslinp /nc. 399 Wes(1200 North, B Orem. U(ah 84057 (80/1235-9741 Far(80I1235-9560 <br />Acute Whole Effluent Toxicity Report <br />Ceriodaphnia <br />DATE: March 22, 2001 <br />PERMITTEE NAME: Western Fuels Co. SITE ID: New Horizon Mine OUTFACE NO: 007 <br />TEST (Anima!/Age): Ceriodaphnia <24 hours. <br />DATE/I'IME TEST BEGAN: 3/15/01 12:30 p.m. <br />SAMPLE (Da(e/Type): 3/14/01 Grab. <br />DATE/I'IME TEST COMPLETED: 3/17/01 1:00 p.m. <br />Ceriodaphnia dubia neonates were exposed to diluted Effluent as specified by the permit. At the end of the test period Survival <br />was measured to determine if Acute Toxicity was present in the sample. <br />Animal Age at Test Start <br />Number of Organisms/Dilution Volume/Replicates <br />Food <br />Aeration <br />Dissolved Oxygen <br />Water Replacement <br />Temperature <br />Photo Period <br />pH <br />Dilution Water <br />Receiving Water <br />Q4 hours. <br />5 organisms/IS mL/4 replicates. <br />Fed YTC and Algae before starting test. <br />None required. <br />Measured daily old/new. <br />Renewed daily. <br />20 ± 1 degrees C. <br />16 hours light 8 hours dark. <br />Measured initially and at 24 hours. <br />Reconstituted lab water approx. 200 mg/L hardness. <br />None was supplied. <br />SUMMARY <br />Results: X Pass Fail <br />There was NO significant effect on survival. <br />Enclosed are data sheets and statistical reports. <br />Sincerely <br />~Li%GIf v`~' c <br />ee Rawlings / <br />Wa[er & Environmental Tes[ing, Inc. <br />Enclosure <br />