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_~ __ .. _ <br />W.E.T. Inc. III IIIIIIIIIIIIIIII <br />Water & Environmental Testing Inc. 399 iy'est IZ00 North. B Orem, Utah 84057 (80//135-9741 Fax/80!1135-9560 <br />Acute Whole Effluent Toxicity Report <br />Fathead Minnow <br />DATE: March 22, 2001 <br />PERMITTEE NAME: Western Fuels Co. SITE ID: New Horizon Mine OUTFACE NO: 007 <br />TEST (Anima!/Age): Fathead Minnow, 7 days. <br />DATE/I'IME TEST BECAN: 3/15/0] 1:00 p.m. <br />TEST CONDITIONS <br />Fathead Minnow larvae were exposed to diluted efFluent as specified by the permit. At the end of the test period Survival was <br />measured to determine if Acute Toxicity was present in the sample. <br />Animal Age at Test Start <br />Number ofOrganisms/Dilution Volume/Replicates <br />Food <br />Aeration <br />Dissolved Oxygen <br />Water Replacement <br />Temperature <br />Photo Period <br />pH <br />SAMPLE (Dote/IypeJ: 3/14/01 Grab. <br />DATE/T1ME TEST COMPLETED: 3/19/01 1:45 p.m. <br />Fathead Minnow, 7 days. <br />10 organisms/150 mL/2 replicates <br />Fed before starting test and at 48 hours, newly hatched <br />brine shrimp. <br />None required. <br />Measured daily old/new. <br />Renewed daily. <br />20 + I degrees C. <br />16 hours light 8 hours dark. <br />Measured initially and at 24 hours. <br />Reconstituted lab water approx. 200 mg/L hardness. <br />Dilution Water <br />Receiving Water <br />Sam le Concenh <br />SUMMARY <br />Results: X Pass Fail <br />There was NO significant effect on survival. <br />Enclosed are data sheets and statistical reports. <br />Sincere) <br />~GG'2~%i%r~~ <br />ee Rawlings <br />Waler & Environmental Testing, Inc. <br />None was supplied. <br />Enclosure <br />