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B. MONITORING REQUIREMENTS PART I Page lh of 19 Permit No. CO-0032115 6. Frequency and Sample Type - Acute WET Testing - 002, 003, 005, 006, 009, 011 The monitoring frequency for acute WET tests shall be quarterly, commencing with the first full calendar quarter following the permit effective date. Quarterly test results shall be reported along with the Discharge Monitoring Report (DMR) submitted for the end of the reporting calendar quarter (i.e., WET testing results for the calendar quarter ending March 31 shall be reported with the DMR due April 28, with the remaining WET testing reports submitted with DI4ts due each July 28, October 28 and January 28). The results shall be submitted on the Acute Toxicity Test report form, available from the Division. Copies of these reports are to be submitted to both [he Division and EPA. The permittee shall conduct each acute WET test in general accordance with methods described in the Division guidance document entitled Guidelines for Conducting Whole Effluent Toxicity Tests. The permittee s a 1 conduct an acute - our WET test using Ceriodaphnia sp., and an acute 96-hour WET test using fathead minnows. Acute tests will be replacement static tests of a single effluent grab sample. 7. Accelerated Testing - 002, 003, 005, 006, 009, 011 If an individual toxicity test shows toxicity at concentrations less than or equal to the Instream Waste Concentration (IWC) of 100 Y, or if SOY, species mortality in any dilution is detected in any acute WET test conducted in accordance with this permit, the permittee shall notify the Division verbally within 24 hours and in writing within 5 days of becoming aware of the toxicity. Within 7 days of becoming aware of the toxicity, the permittee shall then begin accelerated testing of the discharge with whichever species has proven (through the first instance of toxicity) to be the most sensitive. The permittee will continue such testing with one species, once every week until directed by the Division to do otherwise, but in no case for more than five representative tests. 8. Preliminary Toxicity Investigation If directed by the Aivision, the permittee shall perform a preliminary toxicity investigation. The permittee will have 15 working days from the date of Division notification to complete a preliminary toxicity investigation and submit the results to the Division, in writing. The preliminary toxicity investigation may include, but is not limited to: additional chemical and biological monitoring, examination of l pretreatment program records, examination of discharge monitoring J reports, evaluation of treatment processes and chemical use, inspection of material storage and transfer areas to determine if a spill may have occurred, and similar procedures.