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METHODS <br />A grab sample from a naturally occurring spring located above the <br />B seam coal rock inside Somerset Mine was collected on October 13, <br />1992, and received at Commercial Testing and Engineering (CT&E) <br />approximately 24 hours later where it was assigned CT&E number <br />170164. Gunnison River receiving water was also collected and <br />delivered on the same dates. The test was initiated approximately <br />24 hours after collection. <br />Testing was conducted in accordance with the most recent protocols <br />specified by the Water Quality Control Division of the Colorado <br />Department of Health, (2), as outlined in Table 1. <br />Chemical characteristics of th=_ inlet spring and river samples were <br />measured as follows: The pH was measured using a Fisher Scientific <br />model 910 pH meter and ammonia was measured using a LaMOtte <br />ammonia/nitrogen comparator. Total residual chlorine was measured <br />using a LaMotte model STC-C1 colorimeter. Alkalinity and hardness <br />were determined by titrimetric methods specified by the American <br />Public. Health Association (1989). Dissolved oxygen was measured <br />with a YSI.model 54A oxygen meter. <br />The test exposed the cladoceran, Ceriodaphnia dubia, to five <br />concentrations of spring water, 1000, 750, SOo, 12.5s and lo, <br />prepared with Gunnison River water. Control replicates were <br />exposed to 1000 Gunnison River water. <br />Ceriodaphnia survival data failed Shapiro-Wilk's test for <br />normality. Therefore, data was analyzed for statistically <br />significant difference in survival between the control and effluent <br />concentrations using Steel's Many-One Rank test. <br />In addition, the LCSO was determined with the Binomial method. <br />-2- <br />COMMERCIAL TESTING & EN GIN EERI <br />:anal Co OV Wa~erm arFed <br />=nr vom P.o~eu.nn <br />