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ABSTRACT Results from toxicity identification tests, completed on <br />October 9, 1992, suggest that high alkalinity is the cause of <br />Somerset Mine effluent toxicity. Various sources of the toxicant <br />have been considered, one of which is spring water entering the <br />mine above the B seam coal rock. This spring water makes up <br />approximately two thirds of the liquid effluent. About a third of <br />the effluent water comes from groundwater. A sample of the spring <br />water was analyzed and found to have very high alkalinity, <br />(concentrations comparable to that of Somerset effluent). Also, a <br />48 hour acute bioassay found the spring water to be acutely toxic <br />to Ceriodaphnia dubia and to have an LCso of 73%. These findings <br />support the theory that Somerset mine effluent toxicity may be <br />introduced by spring inlet water, and, that toxicity is not a <br />result of effluent treatment. <br />COMMERCIALTESTING 8~ ENGINEERING <br />~n nal Copy lVa~ermarAetl <br />=o. your Prmea~on <br />