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METHODS Cont'd <br />The same amount and normality of acid as above was also added to <br />individual 100e Gunnison River samples, 400 ml each, which served <br />as experimental blanks. Gunnison River and 100°s effluent samples <br />with no acid added served as experimental controls. <br />Mortality after 24 hours was expected in those samples with low pH. <br />This included all the blanks, and, the effluent samples with <br />alkalinities of 95 and <5.0. After verification of the expected <br />mortality, 24 hours following test start, the pH of both the <br />effluent samples above and all the blanks were adjusted to a <br />neutral range with 5 normal and 1.25 normal sodium hydroxide. A 24 <br />hour C. dubia bioassay was then performed using the new pH adjusted <br />samples. <br />The data was analyzed for significant mortality using the Dunnett's <br />method. <br />-3- <br />COMMERCIAL TESTING & ENGINEERING CO. <br />oinal Copy Watermarked <br />-o~ four Pr pienipn <br />