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<br />Table 1 -Colorado Acute Test Conditions <br />Parameter <br />C, dubia & D. magna <br />Fathead Minnow <br />Test Type <br />Temperature <br />Photoperiod <br />Test Chamber Size <br />Test Sample Volume <br />Renewal Period <br />Age of Organisms <br />Number per Chamber <br />Chambers Per Concentration <br />Feeding Regime Per Chamber <br />Test Duration <br />Static Renewal <br />20+/-1°C <br />16 hr IighU8 hr dark <br />30 ml <br />20 ml <br />24 hrs <br />< 24 hrs <br />5 <br />4 <br />None <br />48 hrs <br />Static Renewal <br />20+/-1°C <br />16 hr IighU8 hr dark <br />600 ml <br />300 ml <br />24 hrs <br />4 to 10 days <br />10 <br />4 <br />0.15 ml Brine Shrimp tae hrs) <br />96 hrs <br />Table 2 -Methods and Equipment <br />Characteristic <br />Alkalinity <br />Ammonia, as Nitrogen <br />Chlorine, Total Residual <br />Conductivity <br />Dissolved Oxygen <br />Hardness <br />pH <br />Temperature <br />References <br />MethodlEquipment EPA Method <br />Titrimetric (APHAe, 1989) EPA 310.1 <br />Colorimetrc Analysis Method <br />LaMotte STC-CI Colorimeter <br />Oakton CON 500 Cond. Meter <br />Orion Model 850 DO Meter <br />Titrimetric (APHA, 1989) <br />Fisher Model 925 pH Meter <br />NIST' calibrated thermometer <br />EPA 350.1 <br />EPA 330.4 <br />EPA 120.1 <br />EPA 360.1 <br />EPA 130.2 <br />EPA 150.1 <br />EPA 170.1 <br />American Public Health Association. 1992. Standard Methods for the Examination of Water and <br />Wastewater, 18"' Edition. American Public Health Association, Washington, D.C. <br />Colorado Department of Health. 1998. Guidelines for Conducting Whole Eff-uent Toxicity Tests. Water <br />Quality Control Division, Denver, Colorado 80246-1530. <br />USEPA. 1993. Methods for Measuring the Acute Toxicity of Effluents to Freshwater and Marine <br />Organisms. EPA/600/4-90/027F, 4'" edition. EMSL, Cincinnati, Ohio 45268. <br />e American Public Health Association <br />' National Institute of Standards and Technology <br />FS26 <br />