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<br />Narrative and Discussion <br />PERMIT AND SAMPLE INFORMATION <br />NPDES permit #: <br />Type of sample: <br />Date and time of sample(s): <br />Date and time of test start: <br />Dilution water. <br />Receiving temperature(s): <br />CO-0000213 <br />Effluent Grab <br />011101061200 <br />01/11/06 1130 <br />Reconstituted Laboratory Water <br />3.0 ° C <br />• TEST ORGANISMS <br />Fathead minnow <br />Ceriodaphnia dubia <br />Age: 5 days <br />Age: <24 hrs <br />• PROCEDURE <br />Acute, 48 and 96-hour bioassays as outlined in the most recent protocols specified by the Water <br />Quality Control Division of the Colorado Department of Health (1998). <br />• DISCUSSION <br />According to the Western Fuels -Colorado discharge permit (CO-0000213), an acute WET test is <br />failed whenever the LC~o, which represents an estimate of the effluent concentration which is <br />lethal to 50% of the test organisms in the time period prescribed by the test, is found to be less <br />than or equal to 100°f° effluent. <br />By this definition, the results of this bioassay indicate that effluent from outfall 008 is not acutely <br />toxic to fathead minnow and Ceriodaphnia dubia survival. Therefore, outfall 008 is in compliance <br />with the acute WET portion of its Colorada State Industrial General Minimal Discharge Permit for <br />the fourth quarter of 2006. <br />• DATE OF REPORT <br />January 18, 2006 <br />Report prepared and r view by: Tony Stoyko <br />`" Cry. ' /• /~- 46 <br />Biomoi ' o ng Sy ervisor Date <br /> <br />4 <br />F-526 <br />