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METHODS <br />A two species acute bioassay was conducted with effluent from the West Fork of Sylvester <br />Gulch, outfa11017. An effluent grab sample was collected on August 1, 2000, stored on ice, and <br />delivered to Commercial Testing and Engineering, Co. (CT&E) on August 2, 2000. The effluent <br />sample was received at a temperature of 20.0 ° C and assigned CT&E sample tracking number <br />72-433054. The bioassay was initiated approximately 26 hours after sample collection. <br />Testing was conducted in accordance with the most recent protowls specified by the Water <br />Quality Control Division of the Colorado Department of Health (1998), outlined in Table 1. <br />Basic characteristics of the effluent and dilutions were measured as follows: <br />Characteristic Method/ErEuipment EPA Method <br />Alkalinity <br />Ammonia <br />Chlorine, Total Residual <br />Conductivity <br />Dissolved Oxygen <br />Hazdness <br />pH <br />Temperature <br />Titrimetric (APHAI, 1989) <br />Lachat Analysis Method <br />LaMotte STC-CI Colorimeter <br />Orion Model 160 Cond. Meter <br />Orion Model 850 DO Meter <br />Titrimetric (APHA, 1989) <br />Fisher Model 925 pH Meter <br />IVISTZ calibrated thermometer <br />EPA 310.1 & 130.2 <br />EPA 350.1 <br />EPA accepted <br />EPA 120.1 <br />EPA 360.1 <br />EPA 310.1 & 130.2 <br />EPA 150.1 <br />EPA accepted <br />The tests exposed daphnids, Daphnia magna, to five concentrations of effluent, 100%, 75%, <br />50%, 25%, and 1.9%, prepared using reconstituted laboratory water at 160 mg/L CaC03. <br />Additionally, the tests exposed fathead minnows, Pimephales promelas, to five concentrations of <br />effluent, 100%, 75%, 50%, 25%, and 1.9%, prepazed using reconstituted laboratory water at 115 <br />mg/L CaC03. Control replicates were exposed to 100% laboratory water. <br />The Binomial and Probit methods determined the LCso's, where applicable. Significant <br />differences in mortality were analyzed using Steel's Many-One Rank tests. <br />American Public Health Association <br />s National Institute of Standards and Technology <br />COMMERCIAL TESTING & ENGINEERING CO. <br />F 466 L1E <br />Odginal Watermarked For Your Protection <br />