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SGS <br />Table 1 -Colorado Acute Test Conditions <br />Parameter C. dubia & 0. magna Fathead Minnow <br />Test Type Static Renewal Static Rene~.val <br />Temperature 20 +/- 1 ° C 20 +!- 1 ` C <br />Pholoperiod 16 hr IighUB hr dark 16 hr IighUB hr dark <br />Test Chamber Size 30 ml 600 ml <br />Test Sample Volume 20 ml 300 ml <br />Renewal Period 24 hrs 24 hrs <br />Age of Organisms < 24 hrs 1 to 14 days <br />Number per Chamber 5 10 <br />Chambers Per Concentration 4 4 <br />Feeding Regime Per Chamber 0.1 ml Algae/YTC 0.1 ml Brine Shrimp <br />Test Durahan 48 hrs 96 hrs <br />Table 2 -Methods and Equipment <br />Characteristic Method/Equipment EPA Method <br />Alkalinity Trtrimetnc (APHA°, 1989) EPA 310.1 <br />Ammonia, as Nitrogen Colorimelric Analysis Method EPA 350.1 <br />Chlorine, Total Residual LaMotte STC•CI Colorimeter EPA 330.4 <br />Conductivity Oakton CON 500 Cond. Meter EPA 120.1 <br />Dissolved Oxygen Onon Model 850 DO Meter EPA 360 1 <br />Hardness Titrimetric (APHA, 1989) EPA 130.2 <br />PH Fisher Model 925 pH Meter EPA 150.1 <br />Temperature NIST' calibrated thermometer EPA 170.1 <br />References <br />American Public Health Association. 1992. Standard Methcds for the Examinalivn of Water and <br />Wastewater, 18`" Edition. American Public Health Association, Washington, D.C. <br />Colorado Department o(Health. 1998. Guidelines for Conducting Whole Et77uent Toxicity Tests. Water <br />Quality Control Division, Denver, Colorado 80246-1530. <br />USEPA. 1993. Methods for Measuring the Acute Toxicity o(ENiuents fo Freshwater and Marine <br />Organisms. EPA/60014-90/027F, 4`" edition. EMSL, Cincinnati, Ohio 45268. <br />"Amorlcan Public Health AssociaUOn <br />' National Inslltute of Standards and Technology <br />