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SGS <br />Narrative and Discussion <br />• PERMIT AND SAMPLE INFORMATION <br />NPDES permit #: <br />Type of sample: <br />Date and time of sample(s) <br />Date and time of test start: <br />Dilution water: <br />Receiving temperature(s): <br />TEST ORGANISMS <br />Fathead minnow <br />Daphnia mayna <br />PROCEDURE <br />CO-0000213 <br />Effluent Grab <br />8/31/05 1105 <br />9,'1/05 1220 <br />Reconstituted Laboratory Watei <br />15.0 ° C <br />Age: 6 days <br />Age: <24 hrs <br />Acute, 48 and 96-hour bioassays as outlined in the most recent protocols specified by the Water <br />Quality Control Divison of the Colorado Department of Health (1998). <br />DISCUSSION <br />According to the Nlestern Fuels -Colorado discharge permit (CO-0000213), an acute WET test is <br />failed whenever the LCs;, which represents an estimate of the effluent concentration which is <br />lethal to 50% of the test organisms in the time period prescribed by the lest, is found to be less <br />than or equal to 100°'o effluent. <br />By this definition, the results of this bioassay indicate that effluent from outfall 007 is not acutely <br />toxic to fathead minnow and Daphnia mayna surnval. Therefore, outfall 007 is in compliance <br />with the acute WET portion of its Colorado State Industrial General Minimal Discharge Permit for <br />the third quarter of 2005. <br />DATE OF REPORT <br />September 12, 2005 <br />Repo~rtlpre`p'"ared by: Heath Smith Report reviewed by: Heath Smith <br />r~„~^~ 9~n-oJ ~„~{ n• ~,d x'12-c2r <br />B~omon~toring Supervisor Date B~omorntoring Supervisor Date <br />