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!lETHODS AND MATERIALS _ <br />~) <br />Four replicate Surber samples, each representing an area of 1 ft.2 <br />(0.093m2), were collected at three stations is the Williams Fork River <br />(Figure 1). Station 1 was located approximately 900m upstream from the <br />proposed coal silo; Station 2 was situated approximately 30m below the <br />silo; and Station 3 was located about 1600m downstream from the silo. <br />The stations were characterized as rather shallow (less than lm depth) <br />riffle areas. <br />The Surber sampler was firmly seated on the substrate with the net <br />trailing downstream. All stone and debris is the quadrat were scraped <br />is the mouth of the net. After the coarse material had been cleaned and <br />discarded, the area within the frame was thoroughly stirred with a stick <br />to the maximum possible depth to dislodge the animals inhabiting hyporheic <br />spaces. The contents of the collection net were emptied into a plastic <br />pail filled with sieved water. The contents of the pail were stirred <br />and slowly poured through a No. 30 sieve. The pail was refilled and the <br />process repeated until ao more organisms were present. The material in <br />the sieve was concentrated to one side and washed into a sample jar. <br />All samples were preserved with 10~ buffered formalin/water mixture and <br />labeled properly. <br />Selected chemical and physical parameters were measured concur- <br />really with the macroinvertebrate collections. Temperature, pH, and <br />turbidity were measured in the field. Temperature was measured by the <br />thermistor probe of a YSI Model 54 RC polarigraphic dissolved oxygen <br />meter. Hydrogen ion concentrations (pH) were measured with a corning <br />portable pH meter, model 610A. Turbidity was determined using a Hach <br />2 <br />