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DocuSign Envelope ID: D07924E6-3D94-4398-A066-B683COBAB33E <br /> 4.8 Pimephales promelas Chronic Toxicity Test <br /> TEST: 7-day chronic with Pimephales promelas OPERATORS: LAG, JMS, TE, CL, JW <br /> Start: 1/19/21 12:00 <br /> End: 1/26/21 11:40 <br /> Test Substance: 0.5 g ethanol/g SO4 with/without lime pre-softening <br /> Client/Project: Elk Ridge Mining and Reclamation, LLC <br /> Table 16: Summary results of P.promelas chronic toxicity test. <br /> Treatment 0 100(with 100 (without <br /> %Treated Effluent Lime Pre-softening) Lime Pre-softening) <br /> #alive/#exposed 37/40 18/40 0/40 <br /> %survival 92.5 45 0 <br /> Dry weight(mean wt. (mg)/ 0.400 0.098 0.000 <br /> #exposed±SD) ±0.025 ±0.019 ±0.000 <br /> Dissolved 02 range 6_8 6_5 6_4 <br /> (mg/L) 5.0 2.7 4.0 <br /> pH range(SU) 8_2 8_8 8_8 <br /> 7.9 8.3 8.4 <br /> Conductivity range 376 4330 4090 <br /> (pmho/cm) 319 3760 3900 <br /> Temperature range(°C) 23.9 24.3 24.4 <br /> 22.7 22.7 22.5 <br /> Statistical Analysis: <br /> Survival: <br /> With Lime Pre-softening: (Equal Variance t Two-Sample) = Significant effects (Fail) <br /> Without Lime Pre-softening: (Wilcoxon Rank Sum Two-Sample) = Significant effects <br /> (Fail) <br /> Growth: <br /> With Lime Pre-softening: (Equal Variance t Two-Sample) = Significant effects (Fail) <br /> Without Lime Pre-softening: (Equal Variance t Two-Sample) = Significant effects <br /> (Fail) <br /> GEI Consultants,Inc. 18 February 2021 <br /> Ecological Division Whole Effluent Toxicity Test Report <br /> Stantec-Elk Ridge Mining and Reclamation,LLC <br />