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DocuSign Envelope ID: D07924E6-3D94-4398-A066-B683COBAB33E <br /> 4.7 Pimephales promelas Chronic Toxicity Test <br /> TEST: 7-day chronic with Pimephales promelas OPERATORS: TE, CL, AW, JW, JMS <br /> Start: 11/17/20 15:20 <br /> End: 11/24/20 15:30 <br /> Test Substance: 0.25 g ethanol/g SO4-treated Effluent <br /> Client/Project: Elk Ridge Mining and Reclamation, LLC <br /> Table 15: Summary results of P.promelas chronic toxicity test. <br /> Treatment 0 100 <br /> %Treated Effluent <br /> #alive/#exposed 39/40 38/40 <br /> %survival 97.5 95 <br /> Dry weight(mean wt. (mg)/ 0.362 0.273 <br /> #exposed±SD) ±0.025 ±0.013 <br /> Dissolved 02 range 6_2 5_7 <br /> (mg/L) 5.5 3.5 <br /> pH range(SU) 7_9 8_8 <br /> 7.8 8.5 <br /> Conductivity range 385 4230 <br /> (pmho/cm) 329 3910 <br /> Temperature range(°C) 23.9 24.1 <br /> 22.2 22.7 <br /> Statistical Analysis: <br /> Survival: <br /> (Equal Variance t Two-Sample) = No significant effects (Pass) <br /> Growth: <br /> (Equal Variance t Two-Sample) = Significant effects (Fail) <br /> GEI Consultants,Inc. 17 February 2021 <br /> Ecological Division Whole Effluent Toxicity Test Report <br /> Stantec-Elk Ridge Mining and Reclamation,LLC <br />