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TRE <br />Test Organism <br />14001-436-012 <br />Species <br />Pime hales promelas <br />Age <br />4das <br />Source <br />TRE in-house culture, batch 092615 <br />Acclimation <br />None <br />Feeding <br />0.2 ml of brine shrimp nauplii per test chamber <br />approximately 2 hours prior to 48 hour renewal <br />Reference Toxicant Testing <br />Initiated Se tember 8, 2015 using sodium chloride NaCl <br />TEST RESULTS <br />Biological Data <br />Test Concentration (% Effluent) <br />Percent Survival of Pimephales promelas <br />24 hours 48 hours 72 hours 96 hours <br />0 (Control) <br />100 100 100 100 <br />100 <br />70 45 37.5 25 <br />Control Performance <br />Acceptable <br />Note: See Appendix B for copies of laboratory data sheets <br />Data Analysis and Test Endpoints <br />Biological Endpoint <br />Statistical Endpoint Value (% Effluent) <br />Survival <br />NOAEC <100 <br />96 -hour LCso <100 <br />NOAEC = No Observed Adverse Effect Concentration <br />Note: Analyses completed using, where appropriate, and CETIS version 1.8.7 (2014). <br />Physical and Chemical Data <br />e Temperature in test solutions <br />° Continuous temperature in water bath <br />TRE Environmental Strategies, LLC NELAC Accredited Page 3 of 4 <br />pH <br />Dissolved <br />Conductivity <br />Temperature <br />Treatment (% Effluent) <br />Oxygen (mg/L <br />41S m) <br />°C <br />Low <br />High <br />Low <br />High <br />Low <br />High <br />Low <br />High <br />0 (Control) <br />8.0 <br />8.3 <br />6.8 <br />7.4 <br />315 <br />358 <br />19 <br />20 <br />100 <br />7.9 <br />8.6 <br />6.9 <br />7.3 <br />16,000 <br />17,150 <br />19 <br />20 <br />193 <br />20° <br />Both Treatments <br />7.9 <br />8.6 <br />>_6.8 <br />NA <br />19° <br />20b <br />e Temperature in test solutions <br />° Continuous temperature in water bath <br />TRE Environmental Strategies, LLC NELAC Accredited Page 3 of 4 <br />