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TRE 14001A36-012 <br />Effluent Collection and Receipt <br />Note: See Appendix A for chain of custody records. Although the sample was received within the 35 -hour hold time, <br />the test was initiated beyond the hold time. <br />Effluent Characterization <br />Sample No. <br />pH Hard. (mg/L)' <br />Alk. (mg/L)' <br />Spec. Cond. <br />(µS/cm) <br />Date of <br />Temp. at <br />Sample No. <br />Field No. <br />Collection Date & Time <br />TRE No, <br />0.03 <br />Arrival <br />(m /L <br />(m /L <br />11834 <br />8.3 <br />Receipt <br />64 <br />358 <br /><0.02 <br /><1.0 <br />Daily <br />(11C) <br />1 <br />Not Applicable <br />09/28/15 @ 0815-0817 <br />29059 <br />09/29/15 <br />3.3 <br />Note: See Appendix A for chain of custody records. Although the sample was received within the 35 -hour hold time, <br />the test was initiated beyond the hold time. <br />Effluent Characterization <br />Sample No. <br />pH Hard. (mg/L)' <br />Alk. (mg/L)' <br />Spec. Cond. <br />(µS/cm) <br />TRC b <br />(mg/L) <br />NH, -N <br />(mg/L) <br />1 <br />8.0 340 <br />1,440 <br />17,150 <br />0.03 <br />3.7 <br />As CaCO3 <br />b Total residual chlorine <br />Initial Control Water Characterization <br />Batch No. <br />pH <br />Hard. (mg/L)' <br />Alk. (mg/L)' <br />Spec. Cond. <br />TRC b <br />NH3-N <br />250 ml <br />Replicates per Treatment <br />4 <br />Organisms per Replicate <br />S/cm <br />(m /L <br />(m /L <br />11834 <br />8.3 <br />92 <br />64 <br />358 <br /><0.02 <br /><1.0 <br />'As GaCO3 <br />b Total residual chlorine <br />Test Conditions <br />Type <br />Static -Renewal Acute <br />Test Endpoints <br />Survival <br />Test Chambers <br />500-m1 plastic cups <br />Test Solution Volume <br />250 ml <br />Replicates per Treatment <br />4 <br />Organisms per Replicate <br />10 <br />Test Temperature <br />_ <br />20 t 1°C 5 3°C differential <br />Lighting <br />Fluorescent, 16 hours light: 8 hours dark <br />Chamber Placement <br />Random according to computer-generated chart <br />Aeration? <br />X No Yes <br />Test Solution Renewal <br />Daily <br />TRE Environmental Strategies, LLC NELAC Accredited Page 2 of 4 <br />