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12 J. L. METCALF ET AL. <br />over two -fold and demonstrates the feasibility of molec- <br />ular mining of archived samples. Establishing the tim- <br />ing of human disturbance, coupled with the use of <br />ancient DNA techniques for retrieving phylogenetic <br />information from predisturbance historical samples, <br />paves the way forward for developing conservation <br />goals aimed at restoring threatened and endangered <br />species to their native ranges. <br />Acknowledgements <br />This work was funded by The National Park Service, U.S. Fish <br />and Wildlife Service, U.S. Forest Service, U.S. Bureau of Land <br />Management and Trout Unlimited. We thank the California <br />Academy of Sciences, the Smithsonian Museum of Natural <br />History, the Academy of Natural Sciences in Philadelphia <br />(ANSP), the Museum of Comparative Zoology at Harvard Uni- <br />versity and R. P. Evans at Brigham Young University for access <br />to museum fish samples, and J. Wood for assistance with <br />museum sampling. We are especially grateful to Mark Sabaj <br />Perez and Clare Flemming at ANSP for tracking down letters <br />instrumental in resolving the history of the greenback cutthroat <br />trout type specimens. We thank G. Wilcox and S. Albeke for <br />ArcGIS layers used to develop the figures. We thank V. Kirc- <br />hoff and E. Hekkla for help with initial extraction trials. We <br />thank K. Leitzell, K. Ramirez, A. Hirsch, L. Parfrey, V. McKen- <br />zie, R. Knight, R. Guralnick and J. Zelikova for editorial assis- <br />tance. We thank the Greenback Recovery Team and four <br />anonymous reviewers for feedback that greatly improved this <br />manuscript. The findings and conclusions in this article are <br />those of the authors and do not necessarily represent the views <br />of the U.S. Fish and Wildlife Service. <br />References <br />Allendorf FW, Waples RS (1996) Conservation genetics of Sal - <br />monid fishes. 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