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<br />(. <br /> <br />o <br />..-:,) <br />r_1 <br />~ <br />C <br />Q <br /> <br />( <br /> <br />l. <br /> <br />will accompany the specimens, and the sample number, date, and San Juan River <br />Mile will be inscribed with permanent marker on the outer bag. <br /> <br />All data collected at a habitat will be recorded on one data sheet. The <br />following general habitat information will be collected: 1. Date, 2. Location <br />(River Mile to nearest 0.1 mile), 3. Primary habitat type (Main Channel (MC) or <br />Side Channel (SC) , 4. Specific Habitat Type, S. River Lt or RT, 6. MC Temp (OC) , <br />7. Habitat Temp (OC) , 8. Total Length of Habitat (meters), 9. width (m) @mouth; <br />and average width, 10. Max. Depth of Habitat, 11. Collectors (initials), 12. <br />Landmarks (that might identify particular habitat on aerial photoimagery), 13. <br />Sketch of habitat with and indication of seine haul locations. <br /> <br />The following seine haul information will be collected: <br />1. Sample NO. (suggested starting number: llLV001), 2. Seine used: 1/16" or <br />1/32", 3. Time of haul. 4. Was haul acroSs or parallel to axis of habitat,S. <br />Length of haul (meters), 6. Width of haul (meters). 7. Maximum depth (Dmax) <br />(meters), 8. 10 and 20 Substrate type at Dmax, 9. Depth 'at a point half the <br />distance from Dmax to shore (D,), 10. 10 and 20 Substrate type at D,.ll. Depth at <br />a point half distance from Dmax to other shore (D,I, 12. 10 and 20 Substrate type <br />at D" 13. Mud Depth (a measure of the degree of difficulty seining; 0-4, with <br />4 indicating maximum difficulty), 14. Fish Preserved (Y/N) , 15. No. of Whirlpaks <br /> <br />Following review of this protocol a data sheet will be drafted. <br /> <br />General Sampling Requirements: <br />No. of trips: <br />Duration of each trip: <br />No. of motorized rafts / trip <br />Personnel needed/trip <br /> <br />3 <br />8-9 days <br />2 work boats <br />5 <br /> <br />OVER-WINTER SURVIVAL <br /> <br />Samolino Protocol <br /> <br />Over-winter survival of the early life stage of the target species will be <br />determine by comparing relative densities in fall and spring samples. Sampling <br />protocol closely follows that outlined for larval distribution. Fall and spring <br />sampling will consist of one-trip of effort to characterize the fish community <br />in low velocity habitats. All target species will have reached the juvenile <br />stage and should be identifiable in the field. The sampling protocol for these <br />trips will differ from the larval distribution element in the following manner: <br /> <br />1. Only fish caught in the first haul with the 1/16" mesh seine and the <br />first !laul with the 1/32" mesh seine will be enumerated from each habitat. <br />The second hauls will be searched for target species only. <br /> <br />2. Sample numbers must be unique (suggested starting number: 9lFS001). <br /> <br />General Sampling Requirements: <br />No. of trips: <br />Duration of each trip: <br />No. of motorized rafts / trip <br />Personnel needed/trip <br /> <br />2 <br />8-9 days <br />2 <br />5 <br /> <br />All <br />