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<br />( <br /> <br />Cl <br />.:::> <br />(-.) <br />(:"1 <br />CD <br />~ <br /> <br />( <br /> <br />(. <br /> <br />(rebar) pounded securely into the substrate. Ideally the nets should be <br />submerged an inch or two below the water surface. <br /> <br />Subsequent to each morning's set, drift station personnel will sort through the <br />accumulated debris and preserve all icthyoplankton in lOOt reagent grade ethyl <br />alcohol. Data sheets will accommodate: <br />1. the following general information: date, river mile, weather condition, <br />water temperature at start and pull times, collectors names; and <br />2. information specific to each net set: sample numbers, (each net set <br />will receive a unique sample number, suggest starting with 2aNM001 for the <br />New Mexico station and ~UTOOl for the Mexican Hat station), start time, <br />pull time, sampling effort (minutes), flow meter reading at pull time, <br />volume of H,O sampled, fish sampled {Y/N} , NO. of bottles. <br /> <br />Following review of this protocol a data sheet will be drafted. <br /> <br />Each preserved sample (stored in Mason jars) will be accompanied by a lOOt rag <br />paper label (placed inside jar) with the following information: sample number, <br />date, sample times (start-pull time), collectors initials, indication if there <br />were multiple jars per sample (_of_). Sample number should be written with <br />permanent marker on sample jar top. <br /> <br />Exact net set locations and duration of sets will vary throughout the six week <br />sample period in order to accommodate changing river stage and abundance of <br />drifting fish. <br /> <br />General Sampling Requirements: <br />Drift Stations to be operated daily: <br />Personnel/station: <br /> <br />June 15 - August 15 <br />(2) Bio-Aides <br /> <br />Larva1 Fish Distribution <br /> <br />Samolina Protocol: <br />Conducted concurrently with the larval drift sampling (June 17 - July 26), will <br />be three trips to sample {with seines} the San Juan River fish community in a <br />variety of low velocity habitats. Each trip will start at the Hogback Diversion <br />in New Mexico (RM 177.5) and terminate at or near Clay Hills Crossing (RM 2.5) <br />upstream of Lake Powell. For sampling purposes the river will be sectioned into <br />consecutive 5-mile reaches; one mile from each reach will be sampled (without <br />replacement) during each sampling trip. <br /> <br />Within each sample-mile a variety of low velocity habitats (backwaters, pools, <br />isolated pools, eddies, shorelines, etc.) should be seined; with a target number <br />of four habitats per sample-mile. Two types of seines will be used: 1. 15'x 4' <br />(1/16"" mesh) which is of comparable dimension to those used on the Green and <br />Colorado Rivers, and 2. a one-man, 1.0 m' (1/32" mesh). It is likely YOY target <br />species present in the seined area will be collected with the 1/16"" mesh seine. <br />The purpose of the smaller 1/32" mesh seine is two fold: 1. more accurately <br />characterize the fish community (may collect greater numbers of non-native <br />cyprinid larvae, which may be too small to be effectively sampled with the larger <br />mesh), and 2. to serve as verification that we are adequately sampling target <br />species present. In each habitat two quantified passes will be made with each <br />of the seines, area permitting. If a habitat is too small to accommodate hauls <br />with both types of seines, the one-man seine should be used. <br /> <br />While in the field, the contents of all seine hauls should be searched for target <br />species. Target species should be measured to the nearest millimeter Total <br />Length (TL) and released. All other specimens will either be 1. identified and <br />enumerated as adults or sub-adults, or 2. preserved 'in a lOt buffered formalin <br />solution in WhirlPak containers (double-bagged). A sample label (lOOt rag paper) <br /> <br />A10 <br />