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Last modified
10/24/2016 1:49:03 PM
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3/28/2013 1:44:15 PM
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Animas La Plata Project
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Contents <br />embryos were individually removed by plastic pipette eye droppers. Both live and dead rainbow <br />trout embryos were tallied as they were removed from the picking tray and the live eggs returned <br />to their respective incubation tray. The incubation tray was labeled with the amount of surviving <br />eggs, trial number, and date. <br />96 Hour Delayed Mortality <br />The distinction of whether or not a rainbow trout embryo is dead or not may not be immediately <br />known after the experiment. The stress experienced during sleeve valve passage is momentary <br />but can have a latent effect jeopardizing their survival. Thus, live embryos or embryos appearing <br />to still be alive were placed back into their incubation tray f r 4 +day (96 hour) delayed mortality <br />analysis. Each successive day after sleeve valve passage dead eggs were removed from the <br />incubation tray and tallied. Embryos that retained their natural color, gr;hatched and showed <br />signs of life when prodded were considered as still being alive. Embry that hatched and were <br />partly opaque white and had no movement at 04ere not considered as s I tilli being alive. <br />Delayed mortality analysis was also perfc <br />could be injured and stressed to the eaten <br />majority of fish were immediately killed <br />were injured internally and/or externally: <br />determine if the injuries to the fish woult <br />be able to swim uprigbt,:sei "rely injured <br />on their side but ware still breatd*- Br( <br />determine the surviv "f fish immediate] <br />majority of fish gilling, but not able to he <br />hrs). Some fish; that were able to dd ti <br />were the primary indicators of <br />passage: ]Fish that were abletc <br />typically survived beyond the <br />Sump <br />med on fish that survived sleeve valve passage but <br />where their su al was in jeopardy. While the vast <br />ysleeve valve passage, some managed to survive but <br />Ninet six hour delayed mortality analysis would <br />esult in mortality. White some surviving fish would <br />kh were not able tp hold themselves upright and lay <br />thing,( or Billing) was the minimum criteria used to <br />after -sleeve valve passage. However the vast <br />3 themselves upright, died within 1— 2 days (24 — 48 <br />welves upright after sleeve valve passage ended up <br />dih I wimming, and breathing in an upright position <br />tether or not the fsh was going to survive after sleeve valve <br />eely swim around immediately after sleeve valve passage <br />hr delayed mortality period and were considered as survivors. <br />Hand collection of the t?emaip;% sleeve valve survival test sample in the discharge pipe sump pit <br />is not possible due to safer' ' otors. The discharge pipe sump pit is 16 x 16 ft and the remaining <br />water is too deep to collect`fish and fish embryo samples inside of the sump pit. Further, a trash - <br />rack type grate was installed to prevent entry into this area and can only be removed using a <br />large crane (figure 16). Fish that survive sleeve valve passage could possibly be retained within <br />the sump pit area where they may be able to withstand low water velocities and fish embryos <br />could be suspended in areas where water velocities are not great enough to flush them out. This <br />aspect of not flushing out a portion of the sample from the sump pit was due in part, to testing <br />the sleeve valve in the 10 percent open position (most conservative approach to sleeve valve <br />Page 25 <br />
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