ALP 3

Contents
Post - Experimental Collection
Collection of the test sample after sleeve valve passage occurred immediately after the trial and
within the time period when the 10 in board dam was still retaining water. The entrainment nets
were lifted near the front end and rinsed with a hose washing all of the contents towards the cod
end bucket. The cod end bucket was then pushed forward out of the steel strap and the hose
clamps were loosened and the contents poured and then rinsed into another bucket containing
aerated water. Collection of the test sample upstream of the bulkhead was performed by a single
individual who would carefully enter the spillway area to make sure that no test specimens were
stepped on. Two inches of water remained in the spillway aiea but the depth was shallower near
the sump well pit area. The individual slowly zigzagged througltttut the spillway area collecting
fish and rainbow trout embryos with a soft mesh hand net `and immediately placed them into an
aerated bucket. Multiple passes through the spillway were performed to collect all of the test
specimens. Once the post - experimental collectitttt was complete, all of the buckets were hand
carried to the aquaculture facility and were processed in a shaded environment.
Post - Experimental Analysis
The post - experimental analysis of th+e s,leevo valve passage sample occurred immediately after
the collection process. Each entramment;net 6r ,spillway collec`flctn was emptied into its own 5
gal bucket that had been filled with the vt?' r rematning in the 10 in pool and had an aerator in
operation; this assured'thattltetest sample had adequajte.n ygen and cool water while waiting to
be processed. Each bucket was gently poured iin 4 in deep dlsl white picking tray (9 x 12 in)
and the contents carefully examined;for live fish:;'Ash that were'fbund to be alive after sleeve
valve passage were cou "',0 and their external physlcal condition noted and immediately placed
into a design bucket before r iitming them back,. to their respective 55 gal holding tank.
The holding tank ws.labeled with the number of species surviving sleeve valve passage, trial
number, and date. The training satxxple of fisli'Chat did not survive sleeve valve passage were
enumetted and categorizedinto their "r( pective bodily characteristic; full body, body only, head
only, and part only. Only fish that retained:- -their head to tail characteristics were measured after
sleeve valve paassage whereas, parts or pieces of fish were practically impossible to piece back
together again and were counted?but not re- assembled for measurement. Therefore, we were not
able to account for the total amount of test fish after sleeve valve passage due to the fact that
some fish were destroyed.,tf -the extent that their flesh and major body parts no longer existed.
Rainbow trout embryos used for sleeve valve survival passage were broadly classified as either
being dead or alive once the experimental fish were removed from the picking tray. However,
many rainbow trout embryos were irreparably damaged to the extent where only pieces of shells
remained. The rainbow trout embryos that retained their egg -like characteristics would either
turn completely or partly opaque white when they are dead whereas eggs that were still alive
retained their natural color. Live embryos were removed by carefully spooning them out of the
picking tray and then placing them into a 100 mL beaker (with fresh cool water) and the dead
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