ALP 3

Contents
Water was delivered to each row through a 1 '/z in PVC manifold and to each tank through %
PVC spigots. A 1 in hole was made in each lid for the spigot. The spigots were individually
controlled by PVC ball valves as well as the water pressure for each row of the manifold (figure
7). Water for the 210 gal tanks was delivered by a garden hose line connected to the mainline
manifold and then split 4 -ways into each blue tank. Oxygen was supplied to each row by an
115V/60 Hz outdoor aeration pump and to each tank trough via 1/4 in clear vinyl tubing with 1 '/z
x 1 '/z in Sweetwater® air diffusers controlled by V2 in plastic needle valves. The trash can tanks
were fitted with a 2 in screened bulkhead fitting above the top lip and connected into a 2 in PVC
drainage system which allowed each tank to slowly drain surface water. The larger 210 gal blue
tanks drained through a 1 V2 in standpipe at the center of the tank which was also screened at the
water surface (figure 8). The 2 in PVC drainage system was double - screened at the outlet to
further insure that no live test specimens could escape into Basin Creek.
Figure 7. Air and water individually controlled to each 55 gal round gray holding tank (lids
removed for picture). Two vertical trout incubation trays (8 -tray) at top center of picture.
Figure 8. 'rest fish in 210 gal round polyethylene blue tanks with screened pipe stand (air and
water was temporarily turned off for picture).
At the site, test specimens were transported in 5 -gal plastic buckets that were in new condition
and free of residue or abrasions that may affect the condition or health of the test species. The
buckets were filled with water from the holding tanks and supplied with air from hand -held
aerators. Test specimens were held in the 5 -gal buckets only for short periods of time (< 15
minutes) in effort to minimize stress and changes in water temperature and dissolved oxygen.
Each test group of embryos was held in their own respective container whereas; fish were
divided into groups depending on species and according to their size. The buckets used to
transport fish had a spout -type lid attached to prevent escapement while also providing a hole to
place the aerator hose into the bucket. All plastic buckets were black in color to minimize light
penetration in effort to lessen handling stress during holding and transport.
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