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approximately half <br />of those exceeded <br />the proposed value <br />of 5.9 ug /L, some <br />by greater than <br />ten times. The ef- <br />fect from multiple <br />endocrine disrup- <br />tors, such as the <br />suite of alkylphe- <br />nolic compounds <br />Liquid - liquid extractors studied here, is <br />for identification of trace unknown but stud - <br />organic chemicals ies have indicated <br />an additive effect. <br />Significant treat- <br />ment occurs during infiltration and percolation <br />through the soil absorption system of OWS, <br />though the removal rates and mechanisms of <br />OWCs prior to recharge to the environment are <br />currently unknown. Additionally, there is the <br />potential for treatment failure, by preferential <br />flow through the soil, or from hydraulic failure, <br />such as a storm event, that would send untreated <br />wastewater with pollutant concentrations seen <br />here directly to ground or surface water. Cur- <br />rently, there are no established regulations for <br />these compounds because little is still under- <br />stood about the long -term reproductive effects <br />of chronic exposure to pharmaceutically -active <br />compounds at low concentrations such as those <br />seen here. <br />Of the nine surface waters sampled in the study, <br />very few OWCs were identified, and, if present, <br />were in low concentrations. Cholesterol and 4- <br />methylphenol were the only compounds identi- <br />fied in surface waters above the reporting level. <br />The surface water sites are located in regions <br />which rely heavily on OWS for wastewater treat- <br />ment, but are not directly impacted by wastewa- <br />ter from municipal wastewater treatment plant <br />discharge. Instead, treated OWS effluent perco- <br />lates through the soil until it reaches the water <br />table and recharges the groundwater which may, <br />in turn, recharge the local surface water. Surface <br />water may also be impacted by runoff from agri- <br />culture and development. <br />In the ten ground- <br />water wells, there <br />were more OWCs <br />identified at higher <br />concentrations <br />than in the surface <br />waters. Every <br />groundwater well <br />was contaminated <br />with at least one <br />and at most seven <br />of the 25 OWCs <br />included in this <br />study. Nine of the <br />Surface water site sampled <br />sites are drinking in the study <br />water wells located <br />up gradient on the <br />same property as an OWS included in the study. <br />In the OWS- reliant developments, contamination <br />of these wells could indicate regional impacts to <br />the groundwater from treated effluent recharge. <br />Due to the widespread and growing use of OWS <br />as an appropriate method of wastewater treat- <br />ment, understanding the potential impacts on <br />the receiving environments to which they dis- <br />charge is critical. Results of this study indicate <br />OWCs are present in OWS effluent frequently <br />and in variable concentrations. Treatment oc- <br />curs in the septic tank, in additional pre-treat- <br />ment units such as filters and wetlands, and <br />during percolation through the soil absorption <br />field prior to groundwater recharge. Controlled <br />laboratory- and field -scale transport studies are <br />underway to investigate the key mechanisms of <br />removal during soil treatment. OWCs have been <br />identified in groundwater wells and, to a much <br />lesser extent, in surface waters located in OWS- <br />reliant regions. The results from the transport <br />experiments in conjunction with the occurrence <br />findings will aid in defining potential adverse <br />effects to ecosystem and human health due to or- <br />ganic wastewater contaminants discharge from <br />onsite wastewater treatment systems. <br />Additional funding for this project is provided <br />by Colorado School of Mines. <br />