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A. Task: active pairing of genotyped fish based on interactive database to maintain <br />heterozygosity and all allelic variants present in broodstock. <br />Target date: April 2006 <br />B. Task: monitor production fish and stocked fish annually to ensure no loss of <br />allelic diversity, or no significant change in allelic frequency. <br />Target date: July 2004-July 2008 <br />3) Establish a marking system for all broodstocks to allow identification of each broodfish. <br />Management Action: All broodfish will be pit-tagged. A pedigree database will be <br />established for all broodfish, with a microsatellite genotype of each fish. This database <br />will represent the baseline for broodfish from all facilities. <br />A. Task: Genetic analysis will be performed on all broodfish, beginning with the <br />oldest stocks. <br />Target date: July 2005 <br />B. Task: Paired matings will provide a set of allele frequency expectations of the <br />progeny of each pair based on recorded genotypes. As fish are pooled into <br />populations (ponds for growout), each pond will have a known expected allelic <br />frequency. <br />Target date July 2004-July 2008 <br />C. Task: Fish in grow out ponds will be sampled annually and genetically <br />analyzed to determine if lineage sorting or allelic loss is occurring. If allelic loss is <br />occurring, management plans may be adjusted accordingly. <br />Target date: July 2004-July 2008 <br />33