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DRAFT <br />Missing data <br />300 <br />267 <br />226 <br />7 <br />U <br />O <br />J <br />225 <br />275 <br />234 <br />0.1 <br />o 1 <br /> <br />O,.--11 11111111111-- -- 11 ? I <br />0.05 0.10 0.15 0.20 0.25 <br />Proportion of unresolved alleles <br />3.2 Allele binning <br />I converted the continuous data produced by sizing SSR alleles on an automated sequencer <br />into discrete sizes that correspond to the sizes of repeat units for each locus. This binning <br />process was performed by first plotting the cumulative size-distribution of alleles for each <br />locus. Then I adjusted the starting point for the smallest bin until variation among allele <br />calls within each bin was as small as possible. This adjustment was performed by eye. <br />The binned allele calls are included as a comma separated value file. <br />3.3 Null alleles <br />One consequence of using SSR loci developed for other species is the increased possibility <br />of null alleles. A null allele occurs when the technology developed for one species fails to <br />resolve an allele on another species. This usually results in a situation where individuals <br />appear homozygous for a particular locus when those individuals are actually heterozygous. <br />As a result, even in a randomly mating population, frequencies of genotypes at loci with null <br />4