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<br />and Marsh 1996), but is thought to be at a minimum during the fall and winter <br />months (Douglas and Marsh 1996, Valdez and RyeI1995). If HBC emigrate from <br />the LCR or die between sampling events, it is assumed that both marked and <br />. I <br />unmarked fish are lost at the same rate. The Chapman-Petersen estimator can <br />still be used in this circumstance, but the population estimate will be germane for <br />the population during the marking event. Additionally, if HBC immigrate into the <br />LCR between the two events, then the population estimate will be germane for <br />the population during the recapture event. If both additions and losses (i.e., such <br />as immigration and emigration) occur between the events, there is no possible <br />correction and the estimate will overestimate HBC abundance. Finally, all fish <br />captured during both mark-recapture efforts were handled with utmost care to <br />avoid injury or stress related mortality. <br /> <br />It was not possible to directly test the second assumption that capture and <br />handling during the first event affected the recapture probability in the second <br />event. However, results of the tests examining violation of the third assumption <br />provided indirect evidence of whether the second assumption was violated. <br />Again, careful handling of the fish throughout the study should have minimized <br />problems of violating this assumption. <br /> <br />The third assumption addresses equal capture probability of all fish. This <br />assumption can be violated if the capture gear (i.e., hoop nets) is highly size <br />selective. To determine if the probability of capture varied due to fish size, <br />Kolmogorov-Smirnov tests were applied to the length frequency data collected <br />during both the mark and recapture events. The first test compared the length <br />frequency distributions of marked fish [M] with those captured during the <br />recapture event [C]. The second test compared the length frequency <br />distributions of fish marked during the marking event [M] with those recaptured <br />during the recapture event [R]. Capture probability can also differ by location <br />(i.e., along the LCR river corridor). During marking and recapture events, <br />sampling was equally distributed throughout the entire 13.6 km study area. To <br />validate whether all fish had an equal probability of capture during the marking <br />event regardless of their location, a contingency table analysis was used to test <br />whether the "mark rate" differed among sampling reaches and sub-reaches <br />(Seber 1982). This was performed by dividing the number of recaptured fish [R] <br />by the number of fish captured [C] within each geographic reach, and comparing <br />the results in the contingency table analysis. Similarly, a "recapture rate" can be <br />used to validate whether all fish had an equal probability of capture during the <br />recapture event. This was performed by dividing the number of recaptured fish <br />[R] by the number of fish marked [M] within each geographic reach, and <br />comparing the results in the contingency table analysis. The results the above <br />tests suggested if modifications to the Chapman-Petersen estimator were <br />necessary to minimize bias (Bernard and Hansen 1992). These modifications <br />included stratifying the abundance estimates by length, by geographic reach, or <br />both, if necessary. <br /> <br />The fourth assumption (potential tag loss) has proven to be more problematic <br />to address. During the spring trips of 2001, a dorsal fin punch was used as an <br /> <br />16 <br />