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In summary, the objectives of this project were: <br />• to investigate genetic structure within the Virgin River; <br />• to compare wild and captive populations; and <br />• to assess genetic variation in wild and captive populations. <br />Materials and Methods: <br />Study Area <br />The Virgin River flows across the southern portion of Utah, through Washington <br />County, crosses into Arizona, and into Nevada before ending in Lake Mead. The Moapa <br />River (also known as the Muddy River) is a shorter river that flows in southern Nevada <br />from the Moapa Valley to Lake Mead. Prior to the completion of Boulder Dam in 1935, <br />the Moapa River Joined the Virgin River above the confluence of the Virgin and <br />Colorado Rivers. The construction of the dam inundated the juncture of the Moapa and <br />Virgin Rivers and now both flow separately into the northem arm of Lake Mead. <br />Samples <br />A total of 223 Virgin River chub were analyzed. In 2007, fin clips were collected <br />at several sites along the Moapa and Virgin Rivers by VRRMRP staff (Table 1). Sample <br />sites along the Moapa River included: at or near the Wells Siding Diversion, the power <br />plant, and Glendale Gorge/Narrows. Sample sites in the lower Virgin River included.: at <br />or near Beaver Dam Wash, Arizona; the Bunkerville Diversion, Nevada; and the <br />"experimental reach" (a three to four mile reach between the Mesquite and Bunkerville <br />Diversions) in Nevada. These sample sites were at least 35 miles downriver from the site <br />where fish from Dexter were released in 2006. Sample sites in the upper Virgin