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92) revealed that the Virgin River had 10 alleles not found in the Dexter sample; the <br />Dexter sample had five alleles not found in the Virgin River sample. <br />Population structure <br />The FST values for comparisons between all possible pairs of samples are shown <br />in Table 3. The parameter FST is used to determine genetic divergence based on the null <br />hypothesis that two populations are the same. When differences are detected., tests for <br />statistical significance indicate that there is only a low probability, (e.g. P < 0.05) that <br />the difference in the distribution of genotypes was due to chance. All population pairs <br />were significantly different (P < 0.05 after correction for multiple tests) except the <br />comparison between the Dexter broodstock and Dexter year class 2005-6 (FsT = 0.001, P <br />= 0.402). <br />Comparisons of wild samples that showed the greatest population subdivision <br />were between samples from the Moapa River and each of the Virgin River samples, the <br />upper reach (FST = 0.092, P < 0.001) and the lower reach (FST = 0.126, P < 0.001). There <br />was a low, but statistically significant level of differences between the upper Virgin River <br />sample and the lower Virgin River sample (FsT = 0.020, P< 0.001). Also, there were <br />minor, but statistically significant difference between each of the Dexter samples (DBR <br />and DYC) and each of the samples from the upper and lower Virgin River (FsT ranged <br />from 0.009 to 0.033, P < 0.004). Comparisons between each of the Dexter samples and <br />the sample from the Moapa River indicated a higher level of population subdivision (FsT <br />ranged from 0.098 to 0.118, P < 0.001). <br />14