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<br />studies have shown a strong correlation between survival and size of fish when released. First-year <br />survivorship for fish 30 cm long was 10 percent, compared to 26 percent for fish released at 35 cm <br />(Marsh and others, 2005). The Lake Mohave Native Fish Work Group (NFWG) is an interagency <br />program that is reintroducing razorback suckers in Lake Mohave. They recently recommended <br />increasing the targeted stocking size from 35 to 50 cm for Lake Mohave. Even fish at this size remain <br />vulnerable to large (>20 kg) striped bass (Morone saxatilis) and flathead catfish (Pylodictis olivaris) <br />that are found in the system. There are also other issues in producing larger fish: it increases production <br />costs (per fish) and decreases production numbers. It is unknown whether this change in stocking <br />strategy will help increase populations. <br />Terrestrial researchers have recognized for several decades the importance of survival skills in <br />the animals and birds used in repatriation programs. Current programs not only train, but test the <br />performance of repatriated animals and birds in natural food recognition, foraging ability, and avoidance <br />behavior to predators and humans prior to release. These survival approaches have proven critical in the <br />reintroduction of the gray wolf (Canis lupus), California condor (Gymnogyps california), masked <br />bobwhite quail (Colinus virginianus ridgewayi), and blackfooted ferret (Mustela nigripes) (Ellis and <br />others, 1978; Fritts and others, 1997; Biggins and others, 1998; Bangs and others, J 998). Similar <br />approaches have been adopted by Pacific Northwest salmon augmentation programs. Maynard and <br />Flagg (2001) developed the Natural Rearing System (NATURES) program that incorporates naturaJ <br />feeding, physical conditioning, and predator recognition to improve post-stocking survival. However. <br />similar research or culturing approaches are rare for warm-water species. <br />Mueller and others (2003) found that exercised razorback sucker were less prone to downstream <br />dispersal compared to pond reared fish and Ward and Hilwig (2004) reported bony tail and razorback <br />sucker exercised for two weeks exhibited greater swimming stamina than non-exercised fish. We <br />proposed that if swimming performance was improved, it may also improve predator avoidance skills. <br />The goal of this study was to test whether physical and/or behavioral conditioning would improve <br />predator avoidance skills for bony tail and razorback sucker. <br /> <br />Methods <br /> <br />The study was conducted at Achii Hanyo, a satellite hatchery facility of Willow Beach National <br />Fish Hatchery (Willow BNFH). The facility is located near Parker, Ariz., and is operated by the U.S. <br />Fish and Wildlife Service (FWS). Bony tail and razorback suckers were supplied by Willow BNFH. We <br />used largemouth bass (Micropterus salmoides) as the predator for bony tail and flathead catfish <br />(Pylodictis olivaris) as the predator in razorback sucker experiments. Study predators were collected <br />from the Colorado River and returned unharmed following the study. Surviving native fish were <br />included in native fish stockings elsewhere. <br />The study involved treatment, testing, and observation elements. Treatment activities involved <br />exercising and exposing trial groups to predation prior to the actual predator/prey trials. The swimming <br />performance of exercised and unexercised fish was measured using a flow chamber. Survival <br />performance was determined by placing treated and control groups in large tanks with predators to <br />actually measure predation rates. Working at the fish facility gave us a rare opportunity to spend <br />considerable time observing these fish and conducting impromptu side experiments. Those observations <br />and informal experiments are included in this report. <br /> <br />2 <br />