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12 <br /> <br />2. Perform histopathological studies on vital organs of Colorado <br />squawfish and humpback chubs for the effects of selective toxicants. <br />Acute toxicity of 16 selected chemicals to the Colorado <br />squawfish and humpback chub <br />General Objectives: <br />To determine the relative toxicity of eight organic and eight inorganic <br />toxicants of the Colorado squawfish. <br />Specific Objectives: <br />1. To determine the statistical LC50 and 95% confidence limits of <br />selected chemicals for three life stages (postlarval, juvenile and <br />subadult) of squawfish at a water temperature of 17° C. <br />2. To determine the statistical LC50 and 95% confidence limits of <br />selected chemicals for juvenile squawfish at water temperatures of 12% <br />17° and 22° C. <br />3. To determine the LC50 and 95% confidence limits of selected <br />chemicals for juvenile squawfish at three salinities (Palouse River <br />water, artesian well water, University of Idaho, and simulated Willow <br />Beach water, Arizona) at a water temperature of 17° C. <br />4. To determine the LC50 and 95% confidence limits of selected