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<br />2-12 . Chapter 2 <br /> <br />Marks <br />A Passive Integrated Transponder (PIT) tag was <br />injected into the intra-parietal cavity (Fig. 2-8) of <br />each humpback chub 175 mm TL and longer. <br />Starting in February 1991, minimum size of tagging <br />was reduced to 150 mm TL. External tags (i.e., <br />Carlin or Floy tags) placed by previous investigators <br />were removed from native fish and replaced with <br />PIT tags with both tag numbers recorded. These old <br />tags were replaced at the request of the ACT <br />because PIT tags were considered more reliable; i.e., <br />less chance of tag loss and greater capacity and <br />facility for information retrieval (Burdick and <br />Hamman 1993). PIT tags were also injected into <br />other native species (i.e., tlannelmouth suckers, <br />bluehead suckers) 150 mm TL or greater starting <br />August 1, 1991. <br /> <br />Beginning in January 1993, juvenile humpback chub <br />(range, 60-150 mm TL) were marked with <br />temporary fin punches (Fig. 2-9) to track <br />longitudinal dispersal. A 3-mm diameter biopsy <br />needle was used to punch various fin combinations <br />specific to river subreaches (Wydoski and Emery <br />1983). Various fin punch combinations were used <br />by BIW and AGF for juveniles captured and <br />released within respective subreaches of the <br />mainstem Colorado River (Table 2-3). Also fin clip <br />combinations were used by ASU for juveniles <br />captured and released at respective reaches of the <br />LCR <br /> <br />Final Report <br /> <br /> <br />B <br /> <br />j--I ""F01521 <br />1- 4cm -I <br /> <br />~C <br />em <br /> <br />Fig. 2-8. Attachment sites for Carlin dangler tag (A) <br />and Floy anchor tag (B) by previous investigators, <br />and injection site for PIT tag (C) by this <br />investigation. Approximate fish length = 400 mm TL. <br /> <br />B <br /> <br />c <br /> <br /> <br />o <br /> <br />Fig. 2-9. Juvenile humpback chub with location of <br />scale samples (A), and punches of dorsal fin (B), <br />upper caudal fin lobe (C), and lower caudal fin lobe <br />(D). Approximate fish length = 75 mm TL. <br /> <br />Table 2-3. Fin punch combinations used by BIW and AGF to mark juvenile humpback chub in the mainstem <br />Colorado River, and fin clip combinations used by ASU to mark juveniles in the LCR. <br /> <br />Fin Punch Combinations (BIW, AGF) Location (Colorado River) <br /> <br />dorsal fin <br /> <br />lower caudal fin lobe <br /> <br />upper caudal fin lobe <br />dorsal fin plus upper caudal lobe <br />Fin Clip Combinations (ASU) <br />upper caudal lobe plus right pelvic fin <br />upper caudal lobe plus left pelVic fin <br />lower caudal lobe plus right pelvic fin <br />lower caudal lobe plus left pelvic fin <br /> <br />Malagosa Canyon to Lava Canyon (RM 57.6-65.4) <br />Lava Canyon to Hance Rapid (RM 65.4-76.6) <br />Hance Rapid to Havasu Creek (RM 76.6-156.7) <br />Havasu Creek to Diamond Creek (RM 156.7-225.7) <br /> <br />Location (LCR) <br />Chute Falls to Salt Trail Camp (RK 14.9-10.8) <br />SaltTrail Camp to Sipapu (RK 10.8-7.5) <br />Sipapu to Powell Canyon Camp (RK 7.5-3.0) <br />Powell Canyon Camp to Confluence (RK 3.0-0.0) <br />