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profession (Nickum 1988). These specimens will be provided as reference fish collections for museums or as teaching collections in colleges or universities. 7. If fish are not needed for uses identified in Items 1 through 6, they will be euthanized following the accepted protocol of the fisheries profession (Nickum 1988). (A) These fish may be used to obtain tissues or organs for determining levels of contaminants, age determination from otoliths, or other studies. (B) If the fish are not needed for any of the uses described, they will be discarded using appropriate methods. (C) For instructions on correct procedures, contact the Assistant Regional Director - Law Enforcement, U.S. Fish and Wildlife Service, P.O. Box 25486 - Denver Federal Center, Denver, Colorado 80225. The telephone number for that office is (303) 236-7540 and FAX number is (303) 236-7901. The disposition of wild or captive-reared fish that have died while in captivity will follow the guidance provided in Section 7 (A), (B), and (C). C. Euthanasia Protocol. Euthanasia of excess fish will follow recommendations in the "Guidelines for Use of Fishes in Field Research" (attachment) that were developed jointly by the American Fisheries Society, American Society of Ichthyologists and Herpetologists, and the American Institute of Fisheries Research Biologists (Nickum 1988). D. Additional Requirements: All actions involving the disposition of captive-reared fish must be covered by appropriate Federal and State permits. For instructions on correct procedures, contact the Assistant Regional Director - Law Enforcement, U.S. Fish and Wildlife Service, P.O. Box 25486 - Denver Federal Center, Denver, Colorado 80225. The telephone number for that office is (303) 236-7540 and FAX number is (303) 236- 7901. Also, check with the appropriate State Fish and Wildlife Agency regarding State permit requirements.